[1]谢强,黄作平,刘颖,等.线粒体雌激素受体β通过与Bad相互作用抑制非小细胞肺癌细胞的凋亡[J].南方医科大学学报,2015,(01):98.
点击复制

线粒体雌激素受体β通过与Bad相互作用抑制非小细胞肺癌细胞的凋亡()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年01期
页码:
98
栏目:
出版日期:
2015-01-15

文章信息/Info

Title:
Mitochondrial estrogen receptor β inhibits non-small cell lung cancer cell apoptosis via
interaction with Bad
作者:
谢强黄作平刘颖刘晓黄磊
关键词:
雌激素受体β线粒体非小细胞肺癌BadBax细胞凋亡
Keywords:
estrogen receptor β mitochondria non-small cell lung cancer Bad Bax apoptosis
摘要:
目的探究线粒体雌激素受体β(ERβ)抑制凋亡刺激诱导非小细胞肺癌细胞死亡的分子机制。方法免疫荧光及western
blotting分析细胞内源ERβ的线粒体定位;检测顺铂与十字孢碱(staurosporine,STS)处理过表达或沉默线粒体ERβ后细胞凋亡
的变化;免疫共沉淀和western blotting分析线粒体ERβ与促凋亡蛋白Bad的相互作用。结果ERβ存在于A549及201T细胞的
线粒体中;过表达或沉默ERβ可以显著减少或增加顺铂与STS诱导的Bax激活及细胞凋亡;线粒体ERβ与促凋亡蛋白Bad相互
作用可能阻抑Bax的活化及线粒体转位。结论线粒体雌激素受体β可以通过与Bad相互作用抑制顺铂或STS诱导非小细胞肺
癌细胞的凋亡,提示线粒体ERβ可能是治疗非小细胞肺癌的一个新靶点。
Abstract:
Objective To explore the molecular mechanisms by which mitochondrial estrogen receptor β (ERβ) suppresses
non-small cell lung cancer cell apoptosis induced by apoptotic stimulations. Methods The mitochondrial localization of ERβ in
non-small cell lung cancer cell lines A549 and 201T was determined using immunofluorescence and Western blotting. The
changes of apoptosis of the cells with mitochondrial ERβ overexpression or knockdown in response to cisplatin and STS
treatments were assessed, and mitochondrial ERβ interaction with the pro-apoptotic protein Bad was detected using
co-immunoprecipitation and Western blotting. Results ERβ was localized in the mitochondria in A549 and 201T cells. ERβ
overexpression significantly reduced while ERβ knockdown increased Bax activation and cell apoptosis induced by cisplatin
and STS. Mitochondrial ERβ interaction with pro-apoptotic protein Bad may suppress Bax activation and its translocation to
the mitochondria. Conclusion Mitochondrial ERβ can suppress apoptosis of non-small cell lung cancer cells induced by
cisplatin or STS through interaction with Bad, suggesting the value of mitochondrial ERβ as a new therapeutic target for
treatment of non-small cell lung cancer.

相似文献/References:

[1]王群英,王继德,钟世顺,等.过氧化氢酶对SW480细胞线粒体和凋亡的影响[J].南方医科大学学报,2004,(12):1419.
 WANG Qun-ying,WANG Ji-de,ZHONG Shi-shun,et al.Effects of catalase on the mitochondria and apoptosis of SW480 cells[J].,2004,(01):1419.
[2]吴倩倩,张清华,马莉.人大肠癌线粒体DNA重组质粒的构建[J].南方医科大学学报,2005,(08):1016.
 WU Qian-qian,ZHANG Qing-hua,MA Li.Construction of recombinant eukaryotic expression plasmid pcDNA3.1(+)-mtDNA of human colorectal carcinoma cells[J].,2005,(01):1016.
[3]王玉珏,郭凯,陈驰,等.不同辐射剂量对西藏小型猪小肠线粒体的影响[J].南方医科大学学报,2012,(10):1445.
[4]康敏,李森,钟德君,等.非酒精性脂肪肝大鼠肝细胞凋亡与线粒体膜通透性转换孔开放的关系[J].南方医科大学学报,2013,(07):1062.
[5]张颖,刘宁,任青爱,等.硫氢化钠防治截肢术后大鼠肾脏损伤的机制[J].南方医科大学学报,2013,(08):1146.
[6]张建杏,李红英,周国斌,等.止血带性肢体缺血再灌注损伤后血小板线粒体的变化[J].南方医科大学学报,2014,(07):1075.
[7]王琰,陆园园,鲍维维,等.色霉素A2 诱导人肝癌HepG2 细胞凋亡[J].南方医科大学学报,2014,(10):1449.
[8]许雯,郭予斌,李旭,等.棕榈酸通过肝细胞氧化应激反应激活炎症小体[J].南方医科大学学报,2016,(05):655.
[9]孔令恒,陈玉龙,孙娜,等.抑制CaMKII减轻线粒体氧化应激可改善离体心脏缺血再灌注损伤[J].南方医科大学学报,2018,(02):181.
[10]董合玲,吴洪渊,唐钰,等.AMPK调控不同强度电刺激引起的C2C12肌管线粒体氧化应激[J].南方医科大学学报,2018,(06):742.

更新日期/Last Update: 1900-01-01