[1]张超男,黄学宽,骆言,等.电针对急性痛风性关节炎大鼠踝关节髓样细胞表达激发受体表达的影响[J].南方医科大学学报,2015,(01):133.
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电针对急性痛风性关节炎大鼠踝关节髓样细胞表达激发受体表达的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年01期
页码:
133
栏目:
出版日期:
2015-01-15

文章信息/Info

Title:
Effects of electro-acupuncture on expression of triggering receptor expressed on myeloid
cells 1 in ankle joint synovial tissue of acute gouty arthritis rats
作者:
张超男黄学宽骆言蒋娟万磊王玲
关键词:
急性痛风性关节炎电针髓样细胞表达激发受体1
Keywords:
acute gouty arthritis electro-acupuncture triggering receptor expressed on myeloid cells1
摘要:
目的探讨电针对急性痛风性关节炎(AGA)大鼠受试踝关节滑膜组织髓样细胞表达激发受体(TREM)-1表达的影响。方
法将40只SD雄性大鼠随机分为正常组、模型组、西药组、电针组,每组各10只;正常组常规喂养,其余采用尿酸钠溶液注射法
建立AGA模型。造模前2 d,正常组与模型组按20 ml/kg予以生理盐水灌胃,西药组按1 mg/kg予以秋水仙碱溶液灌胃,电针组
选取受试侧三阴交、解溪、昆仑,频率1.5~2 Hz,电压9V,电流强度1~3 mA,疏密波,留针20 min,1次/d,连续9 d。分析各组大鼠
关节功能障碍,采用ELISA 法检测受试踝关节滑膜组织中肿瘤坏死因子TNF-α、白细胞介素IL-1β的含量,采用免疫组化及
Western blot 法检测TREM-1表达。结果与正常组比较,模型组大鼠功能障碍指数显著增高(P<0.01),TNF-α和IL-1β含量显著
增高(P<0.05),受试踝关节滑膜组织TREM-1表达明显增加(P<0.05);与模型组比较,西药组和电针组大鼠功能障碍指数显著
降低(P<0.01),TNF-α和IL-1β含量显著降低(P<0.05),受试踝关节滑膜组织TREM-1表达明显降低(P<0.05);西药组与电针组
比较,均无统计学意义(P>0.05)。结论电针治疗AGA的机制可能是通过抑制TREM-1的表达。
Abstract:
Objective To investigate the effects of electro-acupuncture (EA) on the expression of triggering receptor expressed on
myeloid cell(TREM)1 in ankle joint synovial tissue of acute gouty arthritis (AGA) rats. Methods Forty male SD rats were
randomly divided into 4 groups: normal, AGA, medication and EA group, 10 rats in each group. AGA model was established by
induced monosodium urate (MSU) method, except the normal group. Tow days before AGA model was established, normal and
AGA groups were lavaged with normal saline (20 ml/kg), medication group was lavaged with colchicine solution (20 ml/kg), EA
(1.5-2Hz, D.-D.wave, 9v, 1-3 mA) was applied to“Sanyinjiao”(SP6),“jiexi”(ST41) and“Kunlun”(BL60) for 20 min, once daily,
continuously for 9 days. Then observed the changes in dysfunction, and the content of TNF-αand IL-1β detected by ELISA ,the
expression of TREM-1 detected by immunohistochemistry and western blot. Results Compared to the normal group, the AGA
group of the dysfunction index increased significantly (P<0.01), the content of TNF-αand IL-1βincreased significantly (P<0.05),
the expression of TREM-1 in synovial tissue increased significantly (P<0.05); the medication and EA groups compared to the
AGA group, the dysfunction index decreased significantly (P<0.01), the content of TNF-αand IL-1βdecreased significantly (P<
0.05), the expression of TREM-1 in synovial tissue decreased significantly (P<0.05); there were not statistically significant
between the medication and EA group (P>0.05). Conclusion EA treating AGA may be through down-regulating the expression
of TREM-1 in synovial tissue.

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更新日期/Last Update: 1900-01-01