[1]陈晓琳,唐成林,谢辉,等.电针对非酒精性脂肪性肝病大鼠肝TLR4、NF-κB表达的影响[J].南方医科大学学报,2014,(11):1584.
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电针对非酒精性脂肪性肝病大鼠肝TLR4、NF-κB表达的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年11期
页码:
1584
栏目:
出版日期:
2014-11-15

文章信息/Info

Title:
Effect of electro-acupuncture on hepatic Toll-like receptor 4 and nuclear factor κB
expressions in rats with non-alcoholic fatty liver disease
作者:
陈晓琳唐成林谢辉唐念珍郜婕刘仁建
关键词:
非酒精性脂肪性肝病电针toll样受体4核因子-κB
Keywords:
non-alcoholic fatty liver disease electro-acupuncture Toll-like receptor 4 nuclear factor κB
摘要:
目的探讨电针对非酒精性脂肪性肝病大鼠肝组织toll样受体4(TLR4)、核因子κB(NF-κB)表达的影响及意义。方法48
只雄性SD大鼠随机分成正常组、模型组、早期干预组、晚期干预组,每组各12只;模型组、早期干预组、晚期干预组均给予高脂饲
料喂养5周建立非酒精性脂肪性肝病模型,在5周末处死每组大鼠各1只,以验证造模成功。早期干预组和晚期干预组均电针
“足三里”、“丰隆”、“三阴交”,1次/d,20 min/次,连续治疗21 d。针刺后检测各组大鼠血清ALT、AST、TC、TG的变化情况,采用
荧光定量PCR和Werstern blot检测大鼠肝脏TLR4和NF-κB的mRNA表达情况。结果模型组大鼠的ALT、AST、TC、TG及肝
脏TLR4、NF-κB的表达均高于其余3 组,差异有统计学意义(P<0.05);针刺后各干预组的ALT、AST、TC、TG及肝脏TLR4、
NF-κB的表达均显著下降,差异有统计学意义(P<0.05)。结论电针“足三里”、“丰隆”、“三阴交”可以调节血脂及肝功,对非酒
精性脂肪肝有一定良性调节作用,其作用机制可能与电针可以调节脂代谢,同时可以下调肝脏组织TLR4、NF-κB的表达,减轻
炎症对非酒精性脂肪性肝病的影响有关。
Abstract:
Objective To investigate the effect of electro-acupuncture on Toll-like receptor 4 (TLR4) nuclear factor κB (NF-κB)
expressions in the liver of rats with non-alcoholic fatty liver disease (NAFLD). Methods SD rat models of NAFLD induced by
feeding high-fat diet for 5 weeks were subjected to early or late intervention with electro-acupuncture at the acupoints Zusanli,
Fenglong and Sanyinjiao for 20 min once daily for 21 consecutive days. The changes in serum ALT, AST, TC, and TG levels
were determined in the rats and hepatic expressions of TLR4 and NF-κB detected with quantitative fluorescence PCR and
Western blotting after treatments. Results Serum ALT, AST, TC, and TG levels and TLR4 and NF-κB levels increased
significantly after NAFLD onset (P<0.05), and were markedly reduced by interventions with electro-acupuncture (P<0.05).
Conclusion Electro-acupuncture at the acupoints Zusanli, Fenglong and Sanyinjiao can modify blood lipid and hepatic
function to produce positive effect on NAFLD possibly by modifying lipid metabolism, lowering expressions of TLR4 and
NF-κB in the liver, and reducing the impact of inflammation on NAFLD.

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更新日期/Last Update: 1900-01-01