[1]夏海坚,刘丹,钟东,等.聚甲基丙烯酸甲酯静电纺丝纳米纤维对大鼠原代星形胶质细胞生长的影响[J].南方医科大学学报,2014,(11):1569.
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聚甲基丙烯酸甲酯静电纺丝纳米纤维对大鼠原代星形胶质细胞生长的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年11期
页码:
1569
栏目:
出版日期:
2014-11-15

文章信息/Info

Title:
Impact of topographic features of electrospun polymethylmethacrylate nanofibers on
growth pattern of rat primary astrocytes
作者:
夏海坚刘丹钟东夏永智晏怡唐文渊孙晓川
关键词:
静电纺丝聚甲基丙烯酸甲酯星形胶质细胞细胞培养接触引导脊髓损伤
Keywords:
electrospining polymethylmethacrylate astrocytes cell culture contact guidance spinal cord injury
摘要:
目的探讨聚甲基丙烯酸甲酯(polymethylmethacrylate, PMMA)电纺纳米纤维的拓扑线索对于大鼠原代星形胶质细胞生
长能力及方式的影响,为脊髓损伤后植入性细胞支架的构建提供前期基础。方法构建具有随机或有序拓扑结构的PMMA电
纺纳米纤维;分离并纯化大鼠原代星形胶质细胞;利用PMMA薄膜作为对照,利用慢病毒技术转染绿色荧光蛋白基因作为显色
手段,分析在星形胶质细胞不同拓扑结构纤维支架上的生长特点。结果随机及有序PMMA电纺纤维均能支持星形胶质细胞
的生长,其拓扑结构能够显著影响星形胶质细胞的生长方式,在有序纤维系统上细胞突起的生长方向能够和基质纤维的延伸方
向保持高度一致;通过绿色荧光蛋白和基质纤维的合成图,发现在两种拓扑结构的纤维系统上,细胞突起均能依附在纤维上向
远处延伸;较之PMMA薄膜,在有序纤维上的星形胶质细胞能生成更长的细胞突起(P<0.01),而在随机纤维上的细胞则形成更
短的突起(P<0.01)。结论PMMA电纺纳米纤维的拓扑结构能够显著影响大鼠原代星形胶质细胞生长能力及方式,其作为植
入性支架具有减轻脊髓损伤后损伤灶胶质瘢痕形成的潜在价值。
Abstract:
Objective To evaluate the impact of the topographic features of electrospun polymethylmethacrylate (PMMA)
nanofibers on growth pattern of primary rat astrocytes. Methods Rat astrocytes were cultured on fabricated random and
aligned electrospun nanofibers. Using PMMA film as the control, the cells were transfected with a lentivirus harboring GFP
gene to reveal the cell growth pattern on different substrates. Results Both random and aligned electrospun PMMA nanofibers
could support the growth of rat astrocytes, but the topographic features of the fibers significantly affected their growth
pattern. On aligned nanofibers, astrocytes extended long cell processes along the direction of the substrate fibers, and on
random fibers the astrocytes formed shorter processes. On merged images of GFP expressions and substrate fibers, the cell
processes of the astrocytes were shown to adhere to and elongate along the fibers both on random and aligned nanofibers.
Conclusion The topographic features of PMMA nanofibers can significantly influence the growth pattern of primary rat
astrocytes. Aligned electrospun nanofibers has the potential to serve as scaffold material for reducing glia scar formation after
spinal cord injury.
更新日期/Last Update: 1900-01-01