[1]王琰,陆园园,鲍维维,等.色霉素A2 诱导人肝癌HepG2 细胞凋亡[J].南方医科大学学报,2014,(10):1449.
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色霉素A2 诱导人肝癌HepG2 细胞凋亡()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2014年10期
页码:
1449
栏目:
出版日期:
2014-10-15

文章信息/Info

Title:
Chromomycin A2 induces apoptosis of HepG2 cells in vitro
作者:
王琰陆园园鲍维维谢美娟杜振宁
关键词:
肝癌色霉素A2细胞凋亡活性氧膜电位线粒体
Keywords:
HepG2 chromomycin A2 apoptosis reactive oxygen species mitochondrial membrane potential mitochondria
摘要:
目的研究色霉素A2对人肝癌细胞HepG2 的凋亡诱导作用,以期为肝癌的治疗提供新的治疗药物。方法MTT法检测
色霉素A2作用HepG2、MCF-7、A549、7901 细胞后对细胞增殖的影响;激光共聚焦显微镜观察HepG2 细胞在色霉素A2(0、
60 nmol/L)的作用下,细胞染色质的变化;色霉素A(2 0、20、40、60 nmol/L)作用HepG2细胞24 h,显微镜观察细胞形态变化,采用
流式细胞术检测细胞凋亡率,活性氧水平及膜电位水平的变化。结果色霉素A2对人肝癌HepG2细胞具有明显的抑制效果,且
呈时间,剂量依赖关系;药物作用细胞后,细胞染色质凝聚,染色加深;细胞形态方面发生细胞皱缩,并且细胞数目变少;流式细
胞仪测定结果显示,细胞凋亡率随药物浓度升高而增大,并且细胞内活性氧水平上升,线粒体膜电位水平降低。结论色霉素A2
诱导人肝癌HepG2细胞产生凋亡,其诱导HepG2细胞凋亡的机制可能与活性氧的升高及线粒体膜损伤有关。
Abstract:
Objective To study the effect of chromomycin A2 in inducing apoptosis of HepG2 cells and explore the molecular
mechanism. Methods HepG2, MCF-7, A549, and 7901 cells were exposed to chromomycin A2 and the changes in the cell
viability were detected using MTT assay. The changes in the chromatins were observed with laser scanning confocal
microscope after incubation of the cells with chromomycin A2 (60 nmol/L) for 24 h. The changes in cell morphology were
examined with a phase-contrast microscope, and the apoptotic cell populations, fluorescent intensity of reactive oxygen
species (ROS) and mitochondrial membrane potential were determined using flow cytometry. Results Chromomycin A2
significantly inhibited the proliferation of the cells in a time- and dose-dependent manner, and caused changes in the cell
morphology and cell apoptosis. Exposure of the cells to chromomycin A2 resulted in chromatin condensation, ROS generation,
and reduction of the mitochondrial membrane potential. Conclusion Increased ROS and mitochondria damage may
importantly contribute to chromomycin A2-induced apoptosis in HepG2 cells.

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更新日期/Last Update: 1900-01-01