[1]顾冬生,熊露,韩艳萍,等.坏死细胞逆转脓毒症小鼠Treg/Th17比例的失衡[J].南方医科大学学报,2014,(07):956.
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坏死细胞逆转脓毒症小鼠Treg/Th17比例的失衡()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年07期
页码:
956
栏目:
出版日期:
2014-07-15

文章信息/Info

Title:
Transfusion of necrotic cells redresses regulatory T cell and Th17 cell imbalance in septic
mice
作者:
顾冬生熊露韩艳萍孙尔维
关键词:
脓毒症调节性T细胞Th17细胞坏死细胞
Keywords:
sepsis regulatory T cells Th17 cells necrotic cells
摘要:
目的研究预输注坏死细胞对脓毒症小鼠模型Treg/Th17细胞平衡的影响。方法将34只C57BL/6小鼠随机分成4组:正
常对照组(PBS组,n=5)、假手术对照组(Sham组,n=5)、脓毒症组(CLP组,n=12)及坏死细胞处理组(Nec组,n=12)。PBS组为
正常小鼠,腹腔注射200 μl PBS一次,Sham组小鼠手术分离盲肠末端后,不进行结扎穿孔;CLP组小鼠用盲肠结扎穿孔法(CLP)
建立脓毒症模型;Nec组小鼠预先腹腔注射2×107个坏死细胞,第5天再行盲肠结扎穿孔。所有小鼠于制备CLP模型后2周处
死,流式细胞仪检测外周血、脾脏和胸腺中CD4+Foxp3+ Treg细胞以及CD4+IL17A+ Th17细胞比例。结果CLP组脾脏中Th17
比例及Treg/Th17比值均显著高于PBS组及sham组(P<0.01);CLP组小鼠胸腺内Treg比例显著低于Sham组(P<0.01);而预输
注坏死细胞,可逆转上述CLP手术后小鼠体内Th17比例、Treg比例及Treg/Th17的比例失衡(P<0.05)。结论预输注坏死细胞
可以逆转脓毒症小鼠体内Treg/Th17细胞亚群的失衡。
Abstract:
Objective To investigate the effect of transfusion of necrotic cells on regulatory T (Treg) and Th17 cell balance in
septic mice. Method Thirty-four C57BL/6 mice were randomized into PBS group (n=5), sham-operated group (n=5), sepsis
group (n=12), and necrotic cell transfusion group (n=12) and subjected to intraperitoneal PBS injection, sham operation by
separating the cecum only, cecal ligation and puncture (CLP), and injection of 2 × 107 necrotic cells 5 days before CLP,
respectively. All the mice were sacrificed 2 weeks after CLP for analyzing the proportion of CD4+Foxp3+Treg cells and CD4+
IL17A+ Th17 cells in the peripheral blood, spleen and thymus by flow cytometry. Results The percentage of Th17 cells and Treg/
Th17 ratio in the spleen was significantly higher in CLP group than in the sham-operated group and PBS group (P<0.01). The
percentage of Treg cells in the thymus was significantly lower in CLP group than in the sham-operated group (P<0.01).
Pre-infusion of necrotic cells redressed the abnormality of Treg and Th17 cell percentages and Treg/Th17 imbalance in mice
following CLP (P<0.05). Conclusion Pre-infusion of necrotic cells can reverse Treg/Th17 imbalance in septic mice.

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更新日期/Last Update: 1900-01-01