[1]钟圣辉,张正刚,傅鸣宇,等.耳蜗核前腹核丛细胞的功能修饰先于听觉的建立[J].南方医科大学学报,2014,(07):923.
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耳蜗核前腹核丛细胞的功能修饰先于听觉的建立()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年07期
页码:
923
栏目:
出版日期:
2014-07-15

文章信息/Info

Title:
Functional refinement of bushy cells in the anterior ventral cochlear nucleus precedes
hearing onset in neonatal rats
作者:
钟圣辉张正刚傅鸣宇杨娟肖中举
关键词:
耳蜗核前腹侧核丛细胞发育电生理
Keywords:
anterior ventral cochlear nucleus bushy cells development electrophysiological property
摘要:
目的通过全细胞膜片钳技术研究新生SD乳鼠耳蜗核前腹核(AVCN)中丛细胞在发育过程中的电生理特性变化。方法
运用全细胞膜片钳记录出生后5~21 d 的SD 乳鼠耳蜗核前腹核中丛细胞的动作电位(AP)反应及微小兴奋性突触后电流
(mEPSC),再通过相关数据采集及分析软件分析动作电位半峰宽和mEPSC的10%~90%上升时间(10%~90% Risetime)及时间
常数,并对此做进一步统计分析处理,进而比较不同年龄段新生SD乳鼠的耳蜗核前腹核中丛细胞的电生理反应特性。结果在
丛细胞动作电位半峰宽的统计分析中发现,丛细胞动作电位的时间精确性随着出生后年龄的增加而增加,在听觉建立(出生后
12 d)附近变化停止。丛细胞mEPSC的时间精确性也随着出生后年龄的增加而增加,在听觉建立附近变化停止。结论耳蜗核
前腹核中丛细胞功能的修饰先于听觉的建立。
Abstract:
Objective To investigate the development of the electrophysiological property of bushy cells in the anterior ventral
cochlear nucleus (AVCN) of neonatal Sprague Dawley (SD) rats. Methods The development of action potential and
spontaneous miniature excitatory postsynaptic currents (mEPSCs) in AVCN bushy cells were investigated by whole-cell
patch-clamp technique in SD rats during the postnatal days 5-21 (P5-21). The half band width of the action potential (AP),
10%-90% risetime and decay tau of the mEPSCs were analyzed. Results The AP of the bushy cells became faster with age from
P5 to P21 and stopped changing around the period of hearing onset, as evidenced by the alteration of half band width of the
AP. The time accuracy of mEPSCs of the bushy cells also increased with age and stabilized around hearing onset as shown by
briefer 10%-90% rise time and decay tau of mEPSCs in P14/P21 than in P7. Conclusion The functional refinement of the bushy
cells in the AVCN precedes hearing onset in neonatal rats.
更新日期/Last Update: 1900-01-01