[1]张雅静,林春水,汪威,等.丙泊酚对荷瘤大鼠肿瘤肺转移及MTA1和Wnt1表达的影响[J].南方医科大学学报,2014,(07):1011.
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丙泊酚对荷瘤大鼠肿瘤肺转移及MTA1和Wnt1表达的影响()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2014年07期
页码:
1011
栏目:
出版日期:
2014-07-15

文章信息/Info

Title:
Effects of propofol on pulmonary metastasis of intravenous injected tumor cells and
expressions of MTA1 and Wnt1 in rats
作者:
张雅静林春水汪威陈莺
关键词:
丙泊酚肿瘤转移MTA1Wnt1
Keywords:
propofol tumor metastasis MTA1 Wnt1
摘要:
目的探讨不同剂量丙泊酚对大鼠MADB106细胞肺转移及肿瘤组织中MTA1和Wnt1表达的影响。方法Fischer344雄
性大鼠40只,随机分为:生理盐水组(S组);脂肪乳剂组(F组);丙泊酚30 mg/kg组(P30组)和50 mg/kg组(P50组),每组10只。
1%戊巴比妥钠50 mg/kg腹腔注射经股静脉分别泵入等容量上述药物,1 h后均静注0.5 ml MADB106肿瘤细胞(2×105个)。3周
后处死,计数肺转移瘤数目;免疫组化检测肺肿瘤组织中MTA1和Wnt1的表达,IPP软件定量分析。结果F组和S组肺转移瘤
数目及肿瘤组织中MTA1和Wnt1的表达无显著差异(P>0.05)。与S组相比,P30和P50组的肺转移瘤数目及肿瘤组织中MTA1
和Wnt1 的表达均显著减少(P<0.01),肺转移瘤数目及MTA1 和Wnt1 的表达与丙泊酚的剂量负相关,Pearson 相关系数分别
为-0.879、-0.980和-0.916(P<0.01)。MTA1和Wnt1的表达呈正相关,Pearson相关系数为0.902(P<0.01)。结论丙泊酚呈剂量
依赖性抑制MADB106肿瘤细胞肺转移,并下调转移瘤中MTA1和Wnt1的表达。
Abstract:
Objective To investigate the effects of different doses of propofol on pulmonary metastasis of intravenous injected
tumor cells and expression of MTA1 and Wnt1 in the metastatic tumor in rats. Methods Forty male Fischer344 rats were
randomly divided into 4 equal groups for intravenous administration of normal saline, intralipid, or propofol at the dose of 30
or 50 mg/kg pumped via the femoral vein. One hour after the infusion, MADB106 tumor cells (2 × 105) were injected
intravenously in the rats. Pulmonary metastasis of the tumor cells was observed and the expression of MTA1 and Wnt1 in the
metastatic tumor detected by immunohistochemistry 3 weeks later. Results The rats receiving saline and intralipid treatments
showed a comparable number of pulmonary metastasis and similar expression levels of MTA1 and Wnt1 in the metastatic
tumor (P>0.05); the tumor number and MTA1 and Wnt1 were significantly lower in the two propofol groups (P<0.01). The
doses of propofol was inversely correlated with the number of pulmonary metastasis (r=-0.879) and expressions of MTA1
(r=-0.980) and Wnt1 (r=-0.916) (P<0.01), and MTA1 and Wnt1 expression levels in the metastatic tumors were closed correlated
(r=0.902, P<0.01). Conclusion Propofol can dose-dependently suppress pulmonary metastasis of intravenously injected tumor
cells and down-regulate MTA1 and Wnt1 expressions in the metastatic tumor tissue.

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更新日期/Last Update: 1900-01-01