[1]陈朝武,周彬,徐莹,等.HBV复制及不同基因表达不影响CDC37水平[J].南方医科大学学报,2014,(06):823.
点击复制

HBV复制及不同基因表达不影响CDC37水平()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年06期
页码:
823
栏目:
出版日期:
2014-06-15

文章信息/Info

Title:
Hepatitis B virus replication and viral gene expressions do not affect CDC37 level in
hepatocytes in vitro
作者:
陈朝武周彬徐莹杨贵奉王战会
关键词:
肝细胞癌CDC37基因表达乙型肝炎病毒
Keywords:
hepatocellular carcinoma CDC37 gene expression hepatitis B virus
摘要:
目的研究HBV复制和不同基因表达是否会影响肝细胞内CDC37的表达水平。方法首先扩增HBV 6种基因P、preS1、
preS2、S、C和X,并构建至pCMV表达载体,分别转染Huh7 和HepG2 细胞系,Western blot 检测CDC37 表达水平;另外将含
CDC37基因启动子序列的荧光素酶报告质粒pGL3与HBV的6种基因表达质粒分别共转染Huh7和HepG2细胞系,检测荧光
素酶信号。其次分别构建B、C、D和CD重组体4种HBV基因型毒株的1.28拷贝全基因质粒,运用AdEasy腺病毒系统进行重组
和包装后分别感染Huh7和HepG2细胞系,并检测CDC37表达水平。结果基因表达结果显示,HBV 6种基因的表达对肝癌细
胞系内CDC37的表达水平无明显影响,而且HBV 6种不同蛋白的表达对CDC37启动子的活性无明显影响。1.28拷贝HBV全
基因质粒的感染结果显示,HBV不同基因型毒株的复制对CDC37基因的表达水平亦无明显影响。结论HBV的复制和不同基
因的表达不会明显影响CDC37的表达水平。
Abstract:
Objective To study the influence of hepatitis B virus (HBV) replication and expressions of different viral genes on
CDC37 level in hepatocytes. Methods We amplified and cloned 6 HBV genes (P, preS1, preS2, S, C and X) into pCMV
expression vectors, which were transfected in Huh7 and HepG2 hepatoma cell lines, and CDC37 expression level in the cells
was detected using Western blotting. W also cloned the promoter sequence of CDC37 into pGL3 vector, and co-transfected
pGL3 with pCMV recombinant plasmids into Huh7 and HepG2 cells and the fluorescent signals were detected. To study the
influence of HBV replication on CDC37 expression, we constructed 1.28-copy overlength genome of HBV genotypes B, C, D
and CD recombinant. The overlength HBV genome was transformed into Adeasier-1 cells for recombination and into 293 cells
for packaging. Huh7 and HepG2 cell lines infected with the packaged HBV recombinant adenoviruses were examined for
CDC37 expression with Western blotting. Results Western blotting showed that the expression of different HBV genes did not
obviously affect the protein level of CDC37 in the hepatocytes. The protein expression of HBV genes had no effect on the
activity of CDC37 promoter. Huh7 and HepG2 cells infected with 1.28-copy HBV replicon showed no significant changes in
the expression level of CDC37. Conclusion HBV replication and its gene expression have no effect on the level of CDC37 in
hepatocytes in vitro.

相似文献/References:

[1]李湘竑,钟克波,刘延,等.索拉菲尼治疗肝癌肝移植术后肿瘤复发患者的疗效及安全性分析[J].南方医科大学学报,2011,(09):1608.
[2]郭丹,陈娜娜,侯连兵,等.前列腺素E2活化EP2受体促进肝癌细胞增殖[J].南方医科大学学报,2011,(09):1564.
[3]陈可和,梁博,邹镇洪,等.Rheb和Rheb’D60K突变基因重组慢病毒载体的构建、鉴定及其对肝癌细胞凋亡的影响[J].南方医科大学学报,2012,(03):341.
[4]赵爱国,吴曙光.沉默CDK7基因对HepG2细胞株的抗增殖作用[J].南方医科大学学报,2005,(01):58.
 ZHAO Ai-guo,WU Shu-guang.Effect of CDK7 gene silencing against hepatoblastoma HepG2 cell proliferation[J].Journal of Southern Medical University,2005,(06):58.
[5]金吴东,陈龙华,穆峰.外源性mTκα基因抑制肝癌细胞HepG2的生长[J].南方医科大学学报,2004,(10):1133.
 JIN Wu-dong,CHEN Long-hua,MU Feng.Exogenous mTκα gene inhibits the growth of HepG2 heptocellular carcinoma cells[J].Journal of Southern Medical University,2004,(06):1133.
[6]余元龙,季明芳,何洁冰,等.紫杉醇、5-氟脲嘧啶抑制肝癌细胞BEL-7402生长和诱导凋亡的比较[J].南方医科大学学报,2005,(07):864.
 YU Yuan-long,JI Ming-fang,HE Jie-bing,et al.Effects of paclitaxel and 5-fluorouracil on growth inhibition and apoptosis of hepatoma cells:a comparative study[J].Journal of Southern Medical University,2005,(06):864.
[7]陈志丰,梁宏,张祥松,等.肝癌治疗后甲胎蛋白增高患者的18F-FDG PET/CT与增强CT对照研究[J].南方医科大学学报,2012,(11):1615.
[8]李超,涂康生,郑鑫,等.MicroRNA-218在肝细胞癌中的表达及功能[J].南方医科大学学报,2013,(08):1127.
[9]黄俊海,周杰.符合Milan标准肝细胞癌患者行肝移植和肝切除术预后分析[J].南方医科大学学报,2014,(03):406.
[10]谢亮海,蒋晗,吴福生.Wnt/β-catenin信号通路在肝细胞癌中的促进作用[J].南方医科大学学报,2014,(06):913.

更新日期/Last Update: 1900-01-01