[1]莫显超,李伟忠.siRNA沉默COX-2基因与增强口腔癌耐药株KB/VCR细胞化疗敏感性的相关性[J].南方医科大学学报,2014,(05):718.
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siRNA沉默COX-2基因与增强口腔癌耐药株KB/VCR细胞化疗敏感性的相关性()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年05期
页码:
718
栏目:
出版日期:
2014-05-15

文章信息/Info

Title:
Small interfering RNA-mediated COX-2 gene silencing enhances chemosensitivity of
KB/VCR cells by suppressing MDR-1 gene expression and P-glycoprotein activity
作者:
莫显超李伟忠
关键词:
口腔肿瘤KB/VCR细胞化疗敏感性P-糖蛋白环氧化酶-2小干扰RNA
Keywords:
oral neoplasm KB/VCR cells chemosensitivity P-glycoprotein cyclooxygenase-2 small interfering RNA
摘要:
目的观察siRNA沉默COX-2基因增强口腔癌耐长春新碱细胞株KB/VCR对长春新碱的敏感性。方法将KB/VCR细
胞分为COX-2 siRNA组、阴性对照组及空白对照组,RT-PCR检测COX-2 mRNA和MDR-1 mRNA的表达,流式细胞术检测细
胞内Rho-123 的蓄积量,MTT法检测细胞活力。结果COX-2 siRNA能增强长春新碱对KB/VCR细胞的生长抑制作用(P<
0.01),同时有效地抑制COX-2 mRNA和MDR-1 mRNA的表达及抑制P-糖蛋白(P-gp)的功能。COX-2 siRNA抑制COX-2基
因表达与抑制P-gp 的外排功能及与抑制MDR-1 mRNA的表达均呈正相关。结论siRNA沉默COX-2 基因可显著增强KB/
VCR细胞对长春新碱的敏感性,其作用与下调节MDR-1基因表达及抑制P-gp功能有关。
Abstract:
Objective To investigate the effect of small interfering RNA (siRNA)-mediated COX-2 gene silencing in enhancing
the chemosensitivity of KB/VCR cell lines. Methods KB/VCR cells were trasnfected with COX-2 siRNA were examined for
expressions of COX-2 and MDR-1 mRNAs with RT-PCR and for Rho-123 accumulation using flow cytometry. MTT assay was
used to analyze the proliferation of the transfected KB/VCR cells. Results Compared with the negative and blank control
groups, COX-2 siRNA transfection resulted in significant growth inhibition of KB/VCR cells exposed to vincristine (P<0.01),
down-regulated the expressions of COX-2 and MDR-1 mRNAs, and obviously increased Rho-123 accumulation in KB/VCR
cells. Conclusion COX-2 gene silencing can enhance the chemosensitivity of KB/VCR cells to vincristine, the mechanism of
which may involve down-regulated MDR-1 gene expression and inhibition of P-glycoprotein activity.

相似文献/References:

[1]廖汶晓,闫怡轩,黄艳青,等.塞来昔布增强口腔癌化疗敏感性与阻滞周期进程的相关性[J].南方医科大学学报,2013,(06):885.

更新日期/Last Update: 1900-01-01