[1]宋国徽,王晋,卢金昌,等.SCUBE3对骨肉瘤细胞功能的影响及其与预后的关系[J].南方医科大学学报,2014,(05):617.
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SCUBE3对骨肉瘤细胞功能的影响及其与预后的关系()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2014年05期
页码:
617
栏目:
出版日期:
2014-05-15

文章信息/Info

Title:
Role of SCUBE3 in promoting osteosarcoma cell growth and its association with
prognosis
作者:
宋国徽王晋卢金昌徐怀远赵志强唐清连邹昌业尹军强谢显彪沈靖南
关键词:
SCUBE3骨肉瘤细胞增殖RNA干扰
Keywords:
SCUBE3 osteosarcoma proliferation RNA interference
摘要:
目的探讨SCUBE3在骨肉瘤细胞和组织中的表达及其与患者预后的关系。方法利用Western blotting技术检测5种骨
肉瘤细胞株SCUBE3 的表达,利用化学合成siRNA 转染骨肉瘤细胞,靶向抑制SCUBE3 表达。CCK8 细胞增殖实验观察
SCUBE3 对U2OS细胞增殖的影响,流式细胞仪检测细胞周期改变。免疫组化检测SCUBE3 在骨肉瘤组织中的表达,并运用
Kaplan-Meier 法进行生存分析。结果SCUBE3 在骨肉瘤细胞株中的表达均高于正常成骨细胞。干扰SCUBE3 表达能抑制
U2OS的生长(P<0.05),同时细胞周期阻滞在G1 期。Kaplan-Meier 生存分析显示,SCUBE3 高表达的患者预后比低表达组差
(P=0.036)。结论SCUBE3在骨肉瘤中高表达,它能促进骨肉瘤细胞的增殖并与患者不良预后相关,提示其可能成为潜在的治
疗靶点。
Abstract:
Objective To detect the expression of SCUBE3 in human osteosarcoma cell lines and surgical specimens of
osteosarcomas and investigate its association with the patients’ prognosis. Methods The expression of SCUBE3 protein was
detected in 5 osteosarcoma cell lines using Western blotting. CCK8 assay was used to assess the effect of SCUBE3 suppression
mediated by two specific small interfering RNAs (siRNAs) on the proliferation of U2OS osteosarcoma cells, and the cell cycle
changes were detected using flow cytometry. Immunohistochemistry was performed to detect the expression of SCUBE3 in 60
osteosarcoma tissues, and Kaplan-Meier method was performed for survival analysis of the patients. Results Compared with
osteoblast hFOB1.19 cells, the osteosarcoma cell lines all showed significantly higher expressions of SCUBE3. In U2OS cells,
suppression of SCUBE3 expression by siRNA significantly inhibited the cell proliferation (P<0.05). Kaplan-Meier survival
analysis indicated that patients with high SCUBE3 expression had worse prognosis than those with low SCUBE3 expression
(P=0.036). Conclusion SCUBE3 is up-regulated in the 5 osteosarcoma cell lines and in primary osteosarcoma tissues to promote
the proliferation of osteosarcoma cells. A high SCUBE3 expression in osteosarcoma tissues is associated with a poor prognosis
of the patients, suggesting that SCUBE3 can serve as a potential therapeutic target for osteosarcoma.

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更新日期/Last Update: 1900-01-01