[1]袁茵,陈丹亮,陈璇,等.人脐带间充质干细胞通过激活Notch信号通路促进白血病细胞株Jurkat的体外存活[J].南方医科大学学报,2014,(04):441.
点击复制

人脐带间充质干细胞通过激活Notch信号通路促进白血病细胞株Jurkat的体外存活()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2014年04期
页码:
441
栏目:
出版日期:
2014-04-15

文章信息/Info

Title:
Human umbilical cord-derived mesenchymal stem cells inhibit
proliferation but maintain survival of Jurkat leukemia cells in
vitro by activating Notch signaling
作者:
袁茵陈丹亮陈璇邵红伟黄树林
关键词:
间充质干细胞脐带白血病增殖Notch信号通路
Keywords:
mesenchymal stem cells umbilical cord leukemia proliferation Notch signaling
摘要:
目的研究人脐带间充质干细胞对白血病细胞株Jurkat体外增殖和存活能力的影响并探讨相关机制。方法组织块贴壁
法分离人脐带间充质干细胞,将其作为基质细胞与Jurkat 细胞进行直接接触共培养。通过免疫磁珠法分离共培养体系中的
Jurkat细胞,流式细胞术检测Jurkat细胞增殖能力、细胞周期和自发凋亡率的变化;Western blotting法检测共培养后Jurkat细胞
内Notch信号通路的活化状况。结果共培养4 d后,Jurkat白血病细胞的增殖减缓、细胞周期被阻滞于G0/G1期,而其自发凋亡
率则出现显著下降,并伴有Notch信号通路靶分子HES-1表达量的增加。结论人脐带间充质干细胞构成的微环境对Jurkat白
血病细胞有保护作用,Notch信号通路可能参与了这一过程。
Abstract:
Objective To investigate the effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on the
proliferation and survival of Jurkat leukemia cells in vitro and explore the possible mechanism. Methods Jurkat leukemia cells
were co-cultured with hUC-MSCs isolated from human umbilical cord tissues by plastic adherence at a ratio of 10∶1. The
proliferation and survival of the co-cultured Jurkat cells, separated by immunomagnetic bead cell sorting on day 4, were
evaluated by flow cytometry. Western blotting was performed to evaluate the activation of Notch signaling in the co-cultured
Jurkat cells. Results Jurkat leukemia cells co-cultured with hUC-MSCs for 4 days showed a lowered proliferation rate and cell
cycle arrest at G0/G1 phase with a reduction in the cell apoptotic rate. Notch signaling pathway was activated in the co-cultured
Jurkat cells as evidenced by an increased cellular expression of HES-1. Conclusion Co-culture with hUC-MSCs can inhibit the
proliferation of Jurkat leukemia cells in vitro and protect the cells from apoptosis by activating Notch signaling, indicating a
potential shielding effect of MSCs on leukemia cells.

相似文献/References:

[1]张俊,谢珊珊,韩晓霞,等.血管内皮生长因子对骨髓源间充质干细胞增殖的影响及其信号机制[J].南方医科大学学报,2011,(10):1697.
[2]陈庆康,田雷容.超声诊断脐带绕头的临床价值(附3例报道)[J].南方医科大学学报,2005,(06):671.
 CHEN Qing-kang,TIAN Lei-rong.Diagnostic value of ultrasound in fetal umbilical cord coiling around the head:report of 3 cases[J].,2005,(04):671.
[3]刘晓静,任高宏,廖华,等.成人骨髓间充质干细胞向成骨细胞定向诱导分化的实验研究[J].南方医科大学学报,2004,(04):408.
 LIU Xiao-jing,REN Gao-hong,LIAO Hua,et al.Induced differentiation of adult human bone marrow derived mesenchymal stem cells in vitro toward osteoblasts[J].,2004,(04):408.
[4]柳太云,张成,肖露露,等.人脐血单个核细胞诱导分化为神经元样细胞[J].南方医科大学学报,2005,(02):152.
 LIU Tai-yun,ZHANG Cheng,XIAO Lu-lu,et al.Induced differentiation of human cord blood monocytes into neuron-like cells in vitro[J].,2005,(04):152.
[5]柳太云,李经伦,姚晓黎,等.3H-TdR标记人骨髓间充质干细胞移植治疗mdx鼠的实验研究[J].南方医科大学学报,2005,(05):498.
 LIU Tai-yun,LI Jing-lun,YAO Xiao-li,et al.Transplantation of 3H-thymidine-labeled human bone marrow-derived mesenchymal stem cells in mdx mice[J].,2005,(04):498.
[6]赵文秀,张磊,刘建明,等.小鼠脂肪间充质干细胞的分离培养及其在肝内归巢[J].南方医科大学学报,2013,(08):1151.
[7]廖军义,周年,林良波,等.联合表达BMP2和Sox9促进体外培养间充质干细胞成软骨分化[J].南方医科大学学报,2014,(03):317.
[8]祝旭龙,颜谭,姚维杰,等.大鼠骨髓间充质干细胞的分离与培养方法优化[J].南方医科大学学报,2014,(11):1621.
[9]聂瑛洁,罗利梅,查艳,等.系统性红斑狼疮患者血浆降低间充质干细胞对B淋巴细胞的抑制作用[J].南方医科大学学报,2016,(08):1090.
[10]卓华丽,白利鹏,刘丹,等.视黄醇对人脐带间充质干细胞表皮生长因子、干细胞因子、集落刺激因子1和白血病抑制因子的影响[J].南方医科大学学报,2017,(02):221.

更新日期/Last Update: 1900-01-01