[1]史鹏伟,高艳彬,卢志阳,等.抗菌肽LL-37对鲍曼不动杆菌生物膜的抑制作用[J].南方医科大学学报,2014,(03):426.
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抗菌肽LL-37对鲍曼不动杆菌生物膜的抑制作用()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年03期
页码:
426
栏目:
出版日期:
2014-03-15

文章信息/Info

Title:
Effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm
作者:
史鹏伟高艳彬卢志阳杨磊
关键词:
抗菌肽LL-37细菌生物膜鲍曼不动杆菌
Keywords:
antibacterial peptide LL-37 biofilm Acinetobacter baumannii
摘要:
目的研究抗菌肽LL-37 对鲍曼不动杆菌生物膜的影响,为将来在临床感染的控制和新抗菌药物的研发提供科学的依
据。方法运用平板结合结晶紫染色法体外建立鲍曼不动杆菌生物膜模型,肉汤稀释法检测LL-37的最小抑菌浓度;采用扫描
电镜和结晶紫染色定量法观察抗菌肽LL-37作用后生物膜结构和量的改变。结果成功体外建立鲍曼不动杆菌生物膜模型,测
得抗菌肽LL-37 MIC为64 μg/ml;经过抗菌肽LL-37作用后,鲍曼不动杆菌成熟生物膜结构被破坏。当LL-37浓度为2.5 μg/ml
时即可破坏生物膜结构,且随着LL-37浓度增加,生物膜的量逐渐减少。结论抗菌肽LL-37在浓度远小于其MIC时即可破坏
鲍曼不动杆菌生物膜结构。
Abstract:
Objective To investigate the effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm.
Methods A model of Acinetobacter baumannii biofilm in vitro was constructed by plates and crystal violet staining method, and
the minimal inhibitory concentration of LL-37 was determined by broth dilution. The biofilm morphology was observed by
scanning electron microscopy and biofilm formation was analyzed by the crystal violet staining of the adherent biofilm in the
presence of antibacterial peptide LL-37. Results In the Acinetobacter baumannii biofilm model, the minimal inhibitory
concentration of LL-37 was 64 μg/ml; LL-37 caused structural damage of the biofilm at a low concentration of 2.5 μg/ml. The
biofilm was decreased gradually as the concentration of LL-37 increased. Conclusion LL-37 even at a concentration far below
its minimal inhibitory concentration can cause structural damage of Acinetobacter baumannii biofilm in vitro.

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更新日期/Last Update: 1900-01-01