[1]廖军义,周年,林良波,等.联合表达BMP2和Sox9促进体外培养间充质干细胞成软骨分化[J].南方医科大学学报,2014,(03):317.
点击复制

联合表达BMP2和Sox9促进体外培养间充质干细胞成软骨分化()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2014年03期
页码:
317
栏目:
出版日期:
2014-03-15

文章信息/Info

Title:
Co-expression of BMP2 and Sox9 promotes chondrogenic differentiation of mesenchymal
stem cells in vitro
作者:
廖军义周年林良波易世雄凡廷旭赵辰胡宁梁熙司维柯黄伟
关键词:
骨形态发生蛋白2Sox9间充质干细胞成软骨分化
Keywords:
bone morphogenetic protein 2 Sox9 mesenchymal stem cells chondrogenic differentiation
摘要:
目的探讨联合表达骨形态发生蛋白2(BMP2)和Sox9对间充质干细胞(MSCs)成软骨分化的影响,为构建组织工程软骨
提供实验依据。方法以小鼠胚胎骨髓MSCs(C3H10T1/2)为种子细胞,重组腺病毒BMP2诱导其分化,联用重组腺病毒Sox9
过表达Sox9信号,重组腺病毒GFP作为对照。Real-Time PCR检测成软骨标志物Ⅱ型胶原(Col2a1)、蛋白聚糖(Aggrecan)、X
型胶原(Col10a1)表达变化;Alcian blue染色了解细胞基质糖胺聚糖合成情况;免疫组化和Western blotting检测Col2a1合成情
况。结果BMP2 能诱导C3H10T1/2 细胞成软骨分化,当与Sox9 联合作用时,Sox9 可促进BMP2 诱导的(Col2a1、Aggrecan、
Col10a1)基因表达上调;促进细胞基质糖胺聚糖、Col2a1蛋白合成。结论BMP2与Sox9联合作用可以促进体外培养MSCs成
软骨分化,可能为组织工程软骨提供新的方法。
Abstract:
Objective To investigate the effect of co-expression of bone morphogenetic protein 2 (BMP2) and Sox9 on
chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro and provide experimental evidence for tissue
engineering of cartilage. Methods Mouse embryonic bone marrow MSC C3H10T1/2 cells were infected with recombinant
adenovirus expressing BMP2, Sox9 and green fluorescent protein (GFP) for 3-14 days, with cells infected with the adenovirus
carrying GFP gene as the control. The mRNA expression of the markers of chondrogenic differentiation, including collagen
type II (Col2a1), aggrecan (ACAN), and collagen type X (Col10a1), were determined by real-time PCR. Alcian blue staining
was used for quantitative analysis of sulfated glycosaminoglycan in the cellular matrix. The expression of Col2a1 protein was
assayed by immunohistochemical staining and Western blot analysis. Results Adenovirus-mediated BMP2 expression induced
chondrogenic differentiation of C3H10T1/2 cells. Overexpression of Sox9 effectively enhanced BMP2-induced expression of the
chondrogenic markers Col2a1, aggrecan and Col10a1 mRNAs, and promoted the synthesis of sulfated glycosaminoglycan and
Col2a1 protein in C3H10T1/2 cells. Conclusion Co-expression of BMP2 and Sox9 can promote chondrogenic differentiation of
MSCs in vitro, which provides a new strategy for tissue engineering of cartilage.

相似文献/References:

[1]魏均强,陈华,郑晓飞,等.hsa-miR-654-5p通过抑制骨形态发生蛋白2调控人骨髓基质干细胞成骨分化[J].南方医科大学学报,2012,(03):291.
[2]刘菲,李学智,付妮妮,等.短刺法对兔膝骨关节炎软骨细胞Sox9、VEGF和ColⅩ表达的影响[J].南方医科大学学报,2016,(07):997.

更新日期/Last Update: 1900-01-01