[1]欧玉荣,康敏,周蕾,等.胃癌中幽门螺杆菌L型感染与MIF、MMP9、VEGF表达的关系[J].南方医科大学学报,2014,(02):180.
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胃癌中幽门螺杆菌L型感染与MIF、MMP9、VEGF表达的关系()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年02期
页码:
180
栏目:
出版日期:
2014-02-15

文章信息/Info

Title:
Infection with L-form of Helicobacter pylori and expressions of MIF, MMP9 and VEGF in
gastric carcinoma
作者:
欧玉荣康敏周蕾承泽农唐素兰于东红
关键词:
胃癌幽门螺杆菌细菌L型巨噬细胞移动抑制因子基质金属蛋白酶-9血管内皮生长因子
Keywords:
gastric carcinoma Helicobacter pylori bacterial L-forms macrophage migration inhibition factor matrixmetalloproteinase 9 vascular endothelial growth factor
摘要:
目的探讨胃癌组织中幽门螺杆菌L型(Hp-L)感染与巨噬细胞移动抑制因子(MIF)、基质金属蛋白酶-9(MMP-9)和血管
内皮生长因子(VEGF)表达之间的相互关系及临床意义。方法应用免疫组织化学染色法及革兰染色检测80例胃癌组织及50
例癌旁正常组织中Hp-L感染情况,同时检测上述组织中MIF、MMP9、VEGF蛋白的表达;运用RT-PCR及Western blotting法检
测30例新鲜胃癌组织及相应切缘组织中MIFmRNA及MIF、MMP9、VEGF蛋白的表达。结果80例胃癌组织中经免疫组化和
革兰染色同时阳性病例有57 例,与对照组相比具有显著性差异(71.25% vs 14%,P<0.05);胃癌组织中Hp-L 阳性组中MIF、
MMP9、VEGF蛋白表达阳性率高于Hp-L阴性组(P<0.05),且Hp-L感染与MIF、MMP9和VEGF表达均呈正相关关系(r=0.598,
r=0.292,r=0.341,P<0.05)。30例胃癌新鲜组织中MIF-mRNA表达(1.583±0.177)高于对照组(1.407±0.078),两者差异具有显著
性(t=3.729,P=0.001);Western blotting法检测MIF、MMP9、VEGF蛋白在胃癌组织中的表达显著高于癌旁正常组织;胃癌组织
中MIF、MMP9均与肿瘤浸润深度及淋巴结转移有关,VEGF蛋白表达与肿瘤浸润深度有关(P<0.05)。结论Hp-L型感染是促
进胃癌的侵袭与转移的重要因素之一,其机制与上调胃癌组织中MIF、MMP9、VEGF表达有关。
Abstract:
Objective To investigate the correlation between infection with L-form of Helicobacter pylori (Hp-L) and the
expressions of macrophage migration inhibition factor (MIF), matrix metalloproteinase 9 (MMP9), and vascular endothelial
growth factor (VEGF) in gastric cancer. Methods Hp-L was examined in 80 gastric carcinoma and 50 adjacent normal tissues
by Gram staining and immunohistochemical staining, and the expressions of MIF, MMP9 and VEGF were detected by
immunohistochemical staining; the expression of MIF mRNA was detected by RT-PCR and the expression of MIF, MMP9 and
VEGF proteins were detected by Western blotting in 30 fresh gastric cancer tissues and the corresponding adjacent tissues.
Results Of the 80 gastric carcinoma tissues, 57 (71.25% ) showed Hp-L positivity detected by both Gram staining and
immunohistochemical staining, as compared with a rate of only 14% in the adjacent normal tissues (P<0.05). The gastric
carcinoma tissues showed higher expression levels of MIF, MMP9 and VEGF proteins than the corresponding adjacent normal
mucosa; the positivity MIF, MMP-9 and VEGF proteins were significantly higher in Hp-L-positive gastric carcinoma than in
Hp-L-negative cases (P<0.05). Positive correlations were found between Hp-L positivity and the expressions of MIF, MMP-9
and VEGF (r=0.598, 0.292, 0.341, respectively, P<0.05). The 30 fresh gastric cancer tissues showed also significantly higher MIF
mRNA expression and MIF, MMP-9 and VEGF protein expressions than the adjacent tissues (t=3.729, P<0.01). The expressions
of MIF and MMP-9 were also related to the clinicopathological factors including lymph node metastasis and depth of invasion
(P<0.05). Conclusion Infection with L-form of Hp-L can be an important factor that contributes to the invasion and metastasis
of gastric carcinoma, the mechanism of which involves up-regulated expressions of MIF, MMP-9 and VEGF.

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更新日期/Last Update: 1900-01-01