[1]许兆忠,贾倩倩,李红瑜,等.小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响[J].南方医科大学学报,2013,(10):1471.
点击复制

小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年10期
页码:
1471
栏目:
出版日期:
2013-10-15

文章信息/Info

Title:
Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells
作者:
许兆忠贾倩倩李红瑜王建成赵艳陈斯佳龙海波
关键词:
小白菊内酯糖尿病肾病核因子-κB单核细胞趋化蛋白-1
Keywords:
parthenolide diabetic nephropathy nuclear factor kappa B monocyte chemoattractant protein-1
摘要:
目的探讨小白菊内酯(PTL)对高糖诱导的大鼠肾小球系膜细胞(MC)增殖、核因子-κB(NF-κB)活性和单核细胞趋化蛋
白-1(MCP-1)表达的影响。方法采用正常葡萄糖组(糖浓度为5.6 mmol/L)和高糖组(糖浓度为30 mmol/L)、高糖+PTL组,分
别体外培养大鼠肾小球MC,MTT法检测MC的增殖,ELISA法检测培养液上清中MCP-1的浓度,Western Blot法检测IκBα反
映NF-κB的活性,EMSA法检测NF-κB的活性。结果高糖诱导后MC增殖、MCP-1表达和NF-κB活性明显增强,NF-κB结合蛋
白IκBα明显降解;应用PTL干预后,MC增殖、MCP-1表达和NF-κB活性均明显降低,IκBα降解被抑制。结论PTL能抑制高糖
诱导的肾小球MC NF-κB活化及MCP-1的表达,从而为PTL用于临床防治糖尿病肾病提供了一定的理论依据。
Abstract:
Objective To explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-κB activation
and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs). Methods MCs were cultured in
normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell
proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IκBα was detected by Western
blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB. Results MC proliferation,
MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBα was
obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose.
Conclusion PTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide
a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.

相似文献/References:

[1]章俊,李萍华,杨蕾,等.高糖通过结缔组织生长因子诱导足细胞减少podocalyxin的表达[J].南方医科大学学报,2011,(05):839.
[2]李沛霖,杨锐,周勇,等.糖尿病肾病患者糖化血清蛋白测定的意义[J].南方医科大学学报,2011,(06):1093.
[3]许兆忠,王明,王艳靖,等.硝基酪氨酸对糖尿病肾病大鼠肾脏NF-κB、MCP-1 及TGF-β1表达的影响[J].南方医科大学学报,2013,(03):346.
[4]方咏红,张继平,周少雄,等.2型糖尿病患者血清血管性血友病因子、血小板活化因子水平与糖尿病肾病的关系[J].南方医科大学学报,2005,(06):729.
 FANG Yong-hong,ZHANG Ji-Ping,ZHOU Shao-xiong,et al.Relationship between serum vWF and PAF in type 2 diabetic patients and diabetic nephropathy[J].Journal of Southern Medical University,2005,(10):729.
[5]江颖娟,薛耀明,张倩,等.Exendin-4对肿瘤坏死因子-α诱导的单核细胞趋化蛋白-1表达的影响[J].南方医科大学学报,2013,(06):930.
[6]陈卫东,常保超,张燕,等.雷公藤多苷对糖尿病大鼠肾组织缺氧诱导因子-1α及内皮素-1表达的影响[J].南方医科大学学报,2015,(04):499.
[7]朱晓娜,王玉环,吴娟娟,等.VEGF及TRPC6的表达与糖尿病肾病大鼠足细胞损伤的相关机制[J].南方医科大学学报,2018,(03):296.
[8]吴凡,陈韵颖,肖花,等.尿液细胞外囊泡中的podocalyxin在糖尿病肾病诊断中的价值[J].南方医科大学学报,2018,(09):1126.[doi:10.12122/j.issn.1673-4254.2018.09.17]
[9]周雪梅,徐从书,王凯,等.益气养阴活血中药对糖尿病肾病大鼠Notch/Hes1 通路与血管内皮CD34、CD144 的影响[J].南方医科大学学报,2019,(07):855.[doi:10.12122/j.issn.1673-4254.2019.07.17]

更新日期/Last Update: 1900-01-01