[1]李超,涂康生,郑鑫,等.MicroRNA-218在肝细胞癌中的表达及功能[J].南方医科大学学报,2013,(08):1127.
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MicroRNA-218在肝细胞癌中的表达及功能()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2013年08期
页码:
1127
栏目:
出版日期:
2013-08-01

文章信息/Info

Title:
MicroRNA-218 expression and its role in hepatocellular carcinoma
作者:
李超涂康生郑鑫张竞拓航高洁姚英民刘青光
关键词:
MicroRNA-218肝细胞癌凋亡增殖Bmi-1CDK6
Keywords:
mircoRNA-218 hepatocellular carcinoma apoptosis proliferation Bmi-1 CDK6
摘要:
目的检测MicroRNA-218(miR-218)在肝细胞癌中的表达情况并研究其在肝癌中的功能。方法收集46例肝细胞癌及对
应癌旁组织,运用qRT-PCR技术检测miR-218 在肝癌及对应癌旁组织中的表达情况;应用miR-218 mimic转染人肝癌细胞系
HepG2,MTT和流式细胞术检测转染后细胞活力和凋亡变化,qRT-PCR和Western blot检测miR-218潜在靶点的表达变化。结
果miR-218在肝癌组织中的表达显著低于对应的癌旁组织(P<0.05);miR-218低表达与肿瘤大小(>5 cm)和高TNM分期(Ⅲ+
Ⅳ)具有显著相关性(P<0.05);在HepG2细胞中,miR-218抑制细胞增殖和诱导凋亡,并下调Bmi-1和CDK6 mRNA及蛋白表达
(P<0.05)。结论miR-218低表达与肝癌的恶性临床病理特征相关,miR-218可能通过下调Bmi-1和CDK6表达抑制肝癌细胞增
殖和促进凋亡。
Abstract:
Objective To investigate the expression of microRNA-218 (miR-218) and its role in hepatocellular carcinoma (HCC).
Methods Forty-six pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-218 expression using
qRT-PCR. A miR-218 mimic was transfected into HepG2 cells, and the cell viability and apoptosis were analyzed by MTT assay
and flow cytometry, and the potential targets of miR-218 were detected by qRT-PCR and Western blotting. Results The
expressions of miR-218 in HCC tissues were significantly down-regulated compared to those in the adjacent tissues (P<0.05).
Down-regulation of miR-218 was found to correlate significantly with the tumor size (>5 cm) and an advanced TNM stage (III+
IV) (P<0.05). Ectopic expression of miR-218 in HepG2 cells resulted in suppressed cell proliferation and enhanced cell
apoptosis as well as the down-regulation of Bmi-1 and CDK6 mRNA and protein expressions (P<0.05). Conclusion The
low-expression of miR-218 is correlated with malignant clinicopathological characteristics of HCC, and miR-218 may inhibit
cell proliferation and promote cell apoptosis by down-regulating Bmi-1 and CDK6 in HCC.

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更新日期/Last Update: 1900-01-01