[1]戴小珍,熊新,王兰,等.CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用[J].南方医科大学学报,2013,(07):994.
点击复制

CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年07期
页码:
994
栏目:
出版日期:
2013-07-15

文章信息/Info

Title:
Effects of CXCR7-shRNA lentiviral vector on the growth and invasiveness of human hepatoma carcinoma cells in vitro
作者:
戴小珍熊新王兰潘克俭何浪李红
关键词:
肝癌CXCR7-shRNA生长侵袭
Keywords:
hepatoma carcinoma CXCR7-shRNA growth invasion
摘要:
目的探讨CXCR7-shRNA慢病毒表达载体靶向抑制CXCR7的表达对人肝癌细胞增殖及侵袭能力的影响。方法利用
RNA干扰技术,构建CXCR7的shRNA慢病毒表达载体,转染人肺转移肝癌细胞株HCCLM3,分别通过RT-PCR和Western blot
检测HCCLM3细胞中CXCR7 mRNA和蛋白质表达水平的变化;然后通过MTT法考察CXCR7沉默对HCCLM3细胞增殖侵袭
能力的影响,通过体外粘附实验和Transwell小室法分别考察CXCR7沉默对HCCLM3细胞粘附及侵袭能力的影响。结果与空
白对照组比较,转染CXCR7-shRNA慢病毒载体的HCCLM3细胞中CXCR7 的mRNA及蛋白水平表达均明显降低(P<0.01),
SDF-1诱导的HCCLM3细胞的增殖能力显著下降(P<0.05),同时HCCLM3细胞的粘附及侵袭能力也明显受到CXCR7-shRNA
的抑制(P<0.01)。结论靶向沉默CXCR7能显著抑制结肝癌细胞的增殖和侵袭能力,为肝癌的治疗提供一个潜在的作用靶点。
Abstract:
Objective To explore the effects of CXCR7 knock-down by CXCR7-shRNA lentiviral vector on the proliferation and
invasion of human hepatoma carcinoma cells in vitro. Methods CXCR7-shRNA lentiviral vector was transfected into
heptatocellular carcinoma HCCLM3 cells. The changes in mRNA and protein expression of CXCR7 in the transfected cells
were investigated using real-time PCR and Western blotting, respectively, and MTT assay was employed to assess the cell
proliferation changes. In vitro adhesion assay and transwell chamber test were used to observe the adhesion and invasiveness
of HCCLM3 cells, respectively. Results Transfection of HCCLM3 cells with CXCR7-shRNA lentiviral vector resulted in a
significantly decreased expression of CXCR7 at both mRNA and protein levels (P<0.01) and obvious suppression of the cell
proliferative activity (P<0.05). CXCR7-shRNA also significantly suppressed the invasiveness and adhesion of HCCLM3 cells (P<
0.01). Conclusion CXCR7 knock-down can significantly inhibit the proliferation and invasiveness of human hepatoma
carcinoma cells in vitro, suggesting the value of CXCR7 as a potential target for hepatoma carcinoma therapy.

相似文献/References:

[1]陈耿臻,韩慧,许铭炎,等. 重组腺病毒ADV-TK 基因的构建及其对肝癌细胞的杀伤作用[J].南方医科大学学报,2010,(08):1887.
 CHEN Geng-zhen,HU Hui,XU Ming-yan,et al. Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells[J].Journal of Southern Medical University,2010,(07):1887.
[2]凌志海,孙权权,张耀伟,等.吉西他滨增强肝癌HepG2细胞体外辐射敏感性的机制研究[J].南方医科大学学报,2011,(12):1993.
[3]陈建发,李宇华,陈引香,等.Apollon siRNA提高肝癌细胞化疗敏感性的实验研究[J].南方医科大学学报,2011,(10):1701.
[4]杨义明,杜钢军,林海红.沙利度胺治疗肝癌的实验研究[J].南方医科大学学报,2005,(08):925.
 YANG Yi-ming,DU Gang-jun,LIN Hai-hong.Experimental study of thalidomide for treatment of murine hepatocellular carcinoma[J].Journal of Southern Medical University,2005,(07):925.
[5]季锡清,李朝龙,杨进城,等.血流阻断的缺血预处理技术在肝癌切除术中的应用[J].南方医科大学学报,2004,(01):66.
 JI Xi-qing,LI Chao-long,YANG Jin-cheng,et al.Application of ischemic preconditioning before hepatic vascular exclusion for resection of hepatocellular carcinoma[J].Journal of Southern Medical University,2004,(07):66.
[6]王素珍,孟维静,安洪庆,等.基于倾向指数匹配法的肝癌病人疗效评价[J].南方医科大学学报,2012,(09):1234.
[7]阳洁,覃贵慧,陈军泽.慢病毒介导shRNA靶向下调Cx26表达对人高侵袭性肝癌细胞上皮间质转化及侵袭的影响[J].南方医科大学学报,2014,(12):1743.
[8]肖芦山,邹雪晶,胡威,等.microRNA-107在肝癌中的表达及临床意义[J].南方医科大学学报,2016,(07):974.
[9]玉艳红,原彤彤,黄力毅,等.外周血单核细胞hFgl2蛋白表达与不同临床类型肝病的关系[J].南方医科大学学报,2013,(03):436.
[10]曲凯,林婷,魏吉超,等.Cisplatin induces cell cycle arrest and senescence via upregulating P53 and P21 expression in HepG2 cells[J].南方医科大学学报,2013,(09):1253.

更新日期/Last Update: 1900-01-01