[1]徐锦程,黄莹莹,李阳,等.siRNA沉默RIP1可增强奥沙利铂诱导口腔癌细胞的凋亡[J].南方医科大学学报,2013,(07):1004.
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siRNA沉默RIP1可增强奥沙利铂诱导口腔癌细胞的凋亡()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年07期
页码:
1004
栏目:
出版日期:
2013-07-15

文章信息/Info

Title:
Small interfering RNA-mediated RIP1 knockdown enhances L-OHP sensitivity of human oral squamous carcinoma cells
作者:
徐锦程; 黄莹莹; 李阳; 浦龙健; 夏飞; 蒋琛琛; 刘浩; 蒋志文
关键词:
口腔癌; RIP1; 小干扰RNA; 奥沙利铂; 凋亡
Keywords:
oral squamous carcinoma;  RIP1;  small RNA interference;  oxaliplatin;  apoptosis
摘要:
目的探讨siRNA沉默受体相互作用蛋白激酶1（RIP1）增强奥沙利铂（L-OHP）诱导口腔癌细胞凋亡的作用，以期为口腔
癌的临床治疗提供新的靶点。方法MTT法检测不同浓度（0、0.25、0.5、1、2、4 μmol/L）L-OHP对口腔癌细胞KB的增殖抑制作
用；Western blotting检测L-OHP（1 μmol/L）处理口腔癌细胞KB不同时间（0、6、16、24 h）RIP1的表达及siRNA转染沉默RIP1口
腔癌细胞KB中RIP1的表达；PI/Annexin V双染法检测L-OHP（1 μmol/L）对siRNA转染沉默RIP1后口腔癌细胞KB凋亡的影
响，同时将未转染和转染空质粒口腔癌细胞KB作为空白和阴性对照。结果1 μmol/L L-OHP 作用于口腔癌细胞KB 24、
48、72 h细胞存活率分别为67.66%、55.17%和41.34%，但24 h细胞凋亡率仅为9.6%。用L-OHP刺激口腔癌细胞KB RIP1的表
达随时间延长不断上升，而siRNA转染沉默RIP1后，RIP1表达明显下降。siRNA转染沉默RIP1后，细胞的凋亡率为9.4%，沉默
RIP1并合用L-OHP进行刺激，细胞的凋亡率为29.1%，明显高于单用L-OHP组。结论抑制RIP1的表达可以增强奥沙利铂诱
导口腔癌细胞的凋亡，为口腔癌的临床治疗提供了新的靶点。
Abstract:
Objective To investigate the effect of small interfering RNA-mediated receptor-interacting protein kinase 1 (RIP1)
knockdown on the sensitivity of human oral squamous carcinoma cells to to oxaliplatin (L-OHP)-induced apoptosis and
explore a new target for clinical treatment of oral squamous carcinoma. Methods The viability of human oral squamous
carcinoma cell line KB exposed to different concentrations (0, 0.25, 0.5, 1, 2, 4 μmol/L) of L-OHP were detected by MTT assay.
PI/Annexin V staining was used to observe cell apoptosis in naive KB cells, cell and transfected with pSH1Si-RIP1 or with the
empty plasmid. Western blotting was used to detect RIP1 expression in KB cells exposed to L-OHP and in cells transfected
with pSH1Si-RIP1. Results Exposure to L-OHP (1μmol/L) for 24, 48, 72 h resulted in KB cell survival rates of 67.66%, 55.17%,
and 41.34% , respectively, but the cell apoptosis rate was only 9.6% following a 24-h exposure. KB cells transfected with
pSH1Si-RIP1 showed an apoptotic rate of 9.4%, which increased to 29.1% following L-OHP exposure. RIP1 expression was first
up-regulated and then down-regulated in KB cells treated with L-OHP, and was significantly reduced after cell transfection
with pSH1Si-RIP1. Conclusion Suppression of RIP1 expression increases the apoptotic rate of human oral squamous
carcinoma cells, suggesting the potential of RIP1 as a new candidate target for clinical treatment of oral squamous carcinoma.

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更新日期/Last Update: 1900-01-01