[1]卢金昌,王晋,雍碧城,等.趋化因子CXCL14在骨肉瘤中的表达及其与预后的关系[J].南方医科大学学报,2013,(06):798.
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趋化因子CXCL14在骨肉瘤中的表达及其与预后的关系()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年06期
页码:
798
栏目:
出版日期:
2013-06-15

文章信息/Info

Title:
Expression of chemokine CXCL14 in primary osteosarcoma and its association with prognosis
作者:
卢金昌王晋雍碧城宋国徽赵志强唐清连邹昌业尹军强谢显彪沈靖南
关键词:
CXCL14骨肉瘤趋化因子
Keywords:
CXCL14 osteosarcoma chenokine
摘要:
目的研究趋化因子CXCL14在骨肉瘤细胞和组织中的表达及其与患者预后的关系。方法利用RT-PCR、酶联免疫吸附
法(ELISA)和荧光定量PCR技术检测4种骨肉瘤细胞株及40对骨肉瘤及癌旁肌肉中CXCL14的表达,CCK8细胞增殖实验、克
隆形成实验观察CXCL14对U2OS细胞增殖的影响,免疫组化检测CXCL14在骨肉瘤组织中的表达,并运用Kaplan-Meier 法进
行生存分析。结果CXCL14在骨肉瘤细胞株中的表达均高于正常成骨细胞,而且其在骨肉瘤组织的表达也显著高于癌旁肌肉
组织(P<0.01)。干扰CXCL14表达能抑制U2OS的生长(P<0.05),经两条CXCL14干扰RNA处理后U2OS克隆形成率分别为
(4.93±0.25)%和(5.03±0.0.31)%,均显著低于阴性对照组(8.03±1.16)%,差异有统计学意义(P<0.05)。Kaplan-Meier生存分析
显示,CXCL14高表达的患者预后比低表达组差(P=0.02)。结论CXCL14在骨肉瘤中高表达,它能促进骨肉瘤细胞的增殖并与
患者不良预后相关,提示其可能成为潜在的治疗靶点。
Abstract:
Objective To detect the expression of CXCL14 in human osteosarcoma cell lines and tissues and investigate its
association with the prognosis of the patients. Methods RT-PCR, enzyme-linked immunosorbnent assay (ELISA) and real-time
PCR were used to detect the expression of CXCL14 in 4 osteosarcoma cell lines and in 40 pairs of osteosarcoma tissues and
adjacent muscular tissues. CCK8 assay and colony formation assay was used to assess the effect of CXCL14 suppression
mediated by two specific siRNAs on the proliferation of U2OS osteosarcoma cells. Immunohistochemistry was performed to
detect the expression of CXCL14 in 58 osteosarcoma tissues, and Kaplan-Meier method and log-rank test were performed for
survival analysis of the patients. Results Significant up-regulation of CXCL14 expression was found in the osteosarcoma cell
lines and in osteosarcoma tissues compared with the adjacent muscles (P<0.01). In U2OS cell, suppression of CXCL14
expression by siRNA significantly inhibited the cell proliferation (P<0.01) and colony formation rate (P<0.05). Kaplan-Meier
survival analysis indicated that patients with high CXCL14 expression had worse prognosis than those with low CXCL14
expression (P=0.02). Conclusion CXCL14 is up-regulated in both osteosarcoma cell lines and primary osteosarcoma tissues to
promote the proliferation of osteosarcoma cells. A high CXCL14 expression in osteosarcoma tissues is associated with a poor
prognosis, suggesting the that CXCL14 serve as a potential therapeutic target for osteosarcoma.

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更新日期/Last Update: 1900-01-01