[1]邵玲巧,王倩倩,郭圆圆,等.NOD/SCID小鼠中A431 侧群与非侧群细胞成瘤组织中EMT相关分子的表达[J].南方医科大学学报,2013,(05):733.
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NOD/SCID小鼠中A431 侧群与非侧群细胞成瘤组织中EMT相关分子的表达()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年05期
页码:
733
栏目:
出版日期:
2013-05-15

文章信息/Info

Title:
Expression of epithelial-mesenchymal transition markers in tumor tissues generated by side population and non-side population of A431 cells in NOD/SCID mice
作者:
邵玲巧王倩倩郭圆圆耿松梅
关键词:
鳞癌侧群细胞上皮间质转化肿瘤干细胞
Keywords:
A431 cells side population cells epithelial-mesenchymal transition cancer stem cells
摘要:
目的旨在分离A431中侧群(SP)细胞及非侧群(NSP)细胞亚群,比较二者在小鼠皮下成瘤能力差异及组织中上皮间质转
化(EMT)相关分子表达。方法培养A431细胞经荧光染料Hoeehst33342染色后,流式细胞仪分选出SP细胞和NSP细胞,接
种到NOD/SCID 小鼠皮下,观察各自成瘤能力,取瘤体组织免疫组织化学染色检测EMT相关分子E-Cadherin、β-catenin、
Vimentin、AXL和Erbb3的表达。结果A431细胞系中成功分选出SP细胞,小鼠皮下成瘤迅速。在SP细胞小鼠成瘤组织周围
细胞中,E-Cadherin、Erbb3表达降低,β-catenin、Vimentin、AXL表达增高。结论A431细胞中含有SP细胞亚群,该亚群细胞具
有较强的肿瘤形成能力,且显示较强的EMT特性。
Abstract:
Objective To isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their
difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers. Methods
A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated
into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL,
and Erbb3 in the tumor tissues were detected by immuohistostaining. Results The tumors generated by SP cells were larger
than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of
tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3
and E-cadherin. Conclusion The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in
tissues.
更新日期/Last Update: 1900-01-01