[1]陈美霓,许静洪,赵菊梅.HSP90抑制剂17-AAG对胃癌SGC-7901细胞生长周期和凋亡的影响[J].南方医科大学学报,2013,(02):271.
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HSP90抑制剂17-AAG对胃癌SGC-7901细胞生长周期和凋亡的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年02期
页码:
271
栏目:
出版日期:
2013-02-01

文章信息/Info

Title:
Effects of HSP90 inhibitor 17-AAG on cell cycle and apoptosis of human gastric cancer cell lines SGC-7901
作者:
陈美霓许静洪赵菊梅
关键词:
17-AAG胃癌SGC-7901细胞凋亡Fas蛋白
Keywords:
17-AAG gastric cancer SGC-7901 cells apoptosis Fas protein
摘要:
目的研究一种特异性的热休克蛋白90抑制剂17-丙烯胺基-17-去甲氧基格尔德霉素(17-AAG)对人胃癌细胞SGC-7901
细胞增殖和凋亡的影响,分析其可能的作用机制。方法应用MTT比色法检测不同浓度的17-AAG对胃癌SGC-7901细胞的生
长抑制率;荧光显微镜下观察PI染色的SGC-7901细胞凋亡形态学变化;采用流式细胞术检测SGC-7901细胞的细胞周期变化
及凋亡率变化;通过免疫组化检测胃癌SGC-7901 细胞中Fas 蛋白的表达。结果MTT 法显示17-AAG 能明显抑制胃癌
SGC-7901 细胞的生长增殖,呈时间、剂量依赖性;流式细胞仪细胞周期分析显示,17-AAG处理48 h后使SGC-7901 细胞发生
G2/M期阻滞,并有诱导SGC-7901细胞凋亡的作用;细胞免疫组化结果显示,SGC-7901细胞胞浆内有Fas表达,随着药物浓度的
加大,阳性表达率也逐渐增加。结论17-AAG 通过改变细胞周期、诱导其凋亡来抑制胃癌SGC-7901 细胞的增殖,上调
SGC-7901细胞中Fas蛋白的表达是诱导凋亡的作用机制之一。
Abstract:
Objective To study the effect of the HSP90 inhibitor, 17-allylamino-17-demethoxygelda-namycin (17-AAG), on cell
proliferation and apoptosis of human cancer SGC-7901 cells and explore the mechanisms. Methods The inhibitory effect of
17-AAG on the proliferation and morphology of SGC-7901 cells was assessed with MTT assay and DNA-PI staining,
respectively. Flow cytometry was employed to analyze the changes in cell cycle and apoptosis of the cells following 17-AAG
exposure. The cellular expression of Fas protein was detected by immunohistochemistry. Results 17-AAG significantly
suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. After treatment with 17-AAG for 48 h,
SGC-7901 cells showed cell cycle arrested at G2/M stage, and the cell apoptosis rate increased with the 17-AAG concentration.
The expression of Fas protein in the cytoplasm of SGC-7901 cells increased gradually with the increase of 17-AAG
concentration. Conclusion 17-AAG can induce apoptosis, alters the cell cycle distribution and up-regulates the expression of
Fas protein in SGC-7901 cells to suppress the cell proliferation.

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更新日期/Last Update: 1900-01-01