[1]郑超,董淑英,童旭辉,等.丙戊酸钠对乳腺癌细胞缝隙连接功能的增强作用及其机制[J].南方医科大学学报,2013,(01):66.
点击复制

丙戊酸钠对乳腺癌细胞缝隙连接功能的增强作用及其机制()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年01期
页码:
66
栏目:
出版日期:
2013-01-15

文章信息/Info

Title:
Functional enhancement of gap junction by valproate acid sodium in breast cancer cells and the mechanism
作者:
郑超董淑英童旭辉蒋国君韩溪
关键词:
乳腺癌丙戊酸钠Cx43缝隙连接
Keywords:
 breast cancer valproate acid sodium connexin43 gap junction
摘要:
:目的探讨丙戊酸钠对乳腺癌细胞Hs578T缝隙连接功能的影响及其可能机制。方法MTT法检测丙戊酸钠的细胞毒
性;细胞接种荧光示踪法测定Hs578T细胞之间荧光传递功能;Western blotting检测丙戊酸钠对Cx43总蛋白表达的影响;细胞
免疫荧光法观察丙戊酸钠对Hs578T细胞膜Cx43蛋白表达的影响。结果MTT检测结果显示丙戊酸钠在0~10 mmol/L浓度范
围内对Hs578T细胞几乎无细胞毒性;细胞接种荧光示踪结果显示丙戊酸钠0~5 mmol/L显著增强Hs578T细胞荧光传递功能
(P<0.01);Western blotting结果显示丙戊酸钠0~5 mmol/L可显著增强Cx43总蛋白表达(P<0.01);细胞免疫荧光结果显示丙戊
酸钠0~5 mmol/L可显著增强Hs578T细胞膜Cx43蛋白的表达。结论丙戊酸钠能够显著增强乳腺癌细胞Hs578T的缝隙连接
功能,这种增强作用可能与其增强Cx43总蛋白和细胞膜Cx43蛋白表达水平有关。
Abstract:
Obective To investigate the effect of valproate acid sodium (VPA) on gap junction intercellular communication in
breast cancer Hs578T cells and explore the mechanism. Methods MTT assay was used to detect the cytotoxicity of VPA on
Hs578T cells, and parachute assay was used to detect the effect of VPA on dye spread of the cells. Western blotting was
employed to detect the expression changes of Cx43 total protein in VPA-treated Hs578T cells. The effect of VPA on the
expression of Cx43 on the surface of Hs578T cells was examined with immunofluorescence assay. Results MTT assay showed
no obvious cytotoxicity of VPA on Hs578T cells at the concentrations below 10 mmol/L. VPA below 5 mmol/L obviously
increased the gap junction function in Hs578T cells (P<0.01), and significantly enhanced the expression of Cx43 total protein (P<
0.01) and Cx43 expression on the surface of Hs578T cells (P<0.01). Conclusion VPA can obviously increase the gap junction
function in Hs578T cells possibly by enhancing Cx43 total protein expression and Cx43 protein expression on the surface of
Hs578T cells.

相似文献/References:

[1]黄伟斌,姚广裕,刘民锋,等.心肌钙蛋白I 对蒽环类化疗的乳腺癌患者心脏毒性的预测价值[J].南方医科大学学报,2011,(06):1047.
[2]郭启帅,黄曦,李少林.沉默PeroxiredoxinⅠ基因对乳腺癌裸鼠移植瘤放射敏感性的影响[J].南方医科大学学报,2011,(07):1119.
[3]冯云,周会行,李军红,等.肝癌缺失基因1和磷酸化粘着斑激酶蛋白在乳腺癌组织中的表达及意义[J].南方医科大学学报,2011,(08):1448.
[4]夏青,史艳侠,刘东耕,等.一项单中心的25例男性乳腺癌临床病理特点总结[J].南方医科大学学报,2011,(09):1469.
[5]李嗣杰,贾泓瑶,吴迪,等.肿瘤翻译调控蛋白在人乳腺癌组织中的表达及临床意义[J].南方医科大学学报,2011,(09):1560.
[6]马吉光,王宁菊,于文洁.“三阴”型与非“三阴”型乳腺癌患者的生物学行为差异性分析[J].南方医科大学学报,2011,(10):1729.
[7]王玲,单保恩,桑梅香,等.靶向沉默p65 基因对人乳腺癌细胞MDA-MB-231增殖及凋亡的影响[J].南方医科大学学报,2011,(10):1742.
[8]陈可和,梁博,邹镇洪,等.Rheb和Rheb’D60K突变基因重组慢病毒载体的构建、鉴定及其对肝癌细胞凋亡的影响[J].南方医科大学学报,2012,(03):341.
[9]胡孝渠,秦利,苏逢锡,等.载体介导HER-2RNA干扰治疗乳腺癌研究[J].南方医科大学学报,2006,(05):570.
 HU Xiao-qu,QIN Li,SU Feng-xi,et al.Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer[J].Journal of Southern Medical University,2006,(01):570.
[10]王晓冰,杨清绪,裴小娟,等.乳腺浸润性癌血管生成相关因子的表达及其临床意义[J].南方医科大学学报,2006,(06):860.
 WANG Xiao-bing,YANG Qing-xu,PEI Xiao-juan Department of Pathology,et al.Expression of angiogenesis-related factors in invasive breast cancer and its clinical significance[J].Journal of Southern Medical University,2006,(01):860.
[11]童旭辉,郑超,蒋国君,等.丙戊酸钠增强阿霉素的体外抗乳腺癌作用[J].南方医科大学学报,2015,(01):62.

更新日期/Last Update: 1900-01-01