[1]李朝旭,唐际存,叶招明.IFN-γ增强人γδT细胞杀伤骨肉瘤细胞的研究[J].南方医科大学学报,2013,(01):22.
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IFN-γ增强人γδT细胞杀伤骨肉瘤细胞的研究()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2013年01期
页码:
22
栏目:
出版日期:
2013-01-15

文章信息/Info

Title:
Interferon-γ enhances human γδ T cell-mediated osteosarcoma cell killing in vitro
作者:
李朝旭唐际存叶招明
关键词:
免疫治疗骨肉瘤干扰素-γγδ T细胞
Keywords:
immunotherapy osteosarcoma interferon-γ γδ T cells
摘要:
目的研究干扰素-γ(IFN-γ)增强骨肉瘤患者γδ T细胞对骨肉瘤杀伤作用的影响。方法以唑来磷酸法扩增骨肉瘤患者外
周血γδ T细胞。分别使用实时荧光定量聚合酶链反应和流式细胞术检测IFN-γ处理前后骨肉瘤细胞Fas的表达情况;LDH法检
测骨肉瘤患者γδ T细胞对骨肉瘤细胞的杀伤活性。结果IFN-γ对骨肉瘤细胞Fas的表达明显提高(P<0.01),骨肉瘤患者γδ T细
胞对骨肉瘤细胞的杀伤作用显著增强(P<0.01)。抗人FasL抗体对γδ T细胞杀伤OS细胞作用影响较小(P>0.05);但能显著阻断
γδ T细胞杀伤IFN-γ预处理OS细胞的作用,差异均有统计学意义(P<0.01)。结论IFN-γ可增强骨肉瘤患者γδ T细胞的抗骨肉
瘤作用。
Abstract:
Objective To investigate the cytotoxic effect of γδ T cells from osteosarcoma patients against interferon-γ
(IFN-γ)-treated osteosarcoma cells in vitro. Methods Human γδ T cells were amplified by zoledronate from peripheral blood
cells of osteosarcoma patients. The expression of Fas on the osteosarcoma cells were measured by flow cytometry and
quantitative real-time PCR analysis before and after IFN-γ treatment. The cytoxicity of γδ T cells against osteosarcoma cells
was evaluated with LDH assay. Results IFN-γ significantly enhanced the susceptibility of the osteosarcoma cell lines HOS and
U2OS to the cycotoxicity of γδ T cells from osteosarcoma patients (P<0.01). IFN-γ obviously up-regulated the expression of Fas
in HOS and U2OS cells (P<0.01). Anti-FasL mAb failed to inhibit the cytotoxicity of γδ T cells in untreated osteosarcoma targets
(P>0.05), but significantly impaired γδ T cell cytotoxicity in IFN-γ pre-treated osteosarcoma targets (P<0.01). Conclusion
IFN-γ can enhance the cytotoxic effect of human γδ T cells from osteosarcoma patients against osteosarcoma cells in vitro.

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更新日期/Last Update: 1900-01-01