[1]王玉珏,郭凯,陈驰,等.不同辐射剂量对西藏小型猪小肠线粒体的影响[J].南方医科大学学报,2012,(10):1445.
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不同辐射剂量对西藏小型猪小肠线粒体的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2012年10期
页码:
1445
栏目:
出版日期:
2012-10-01

文章信息/Info

作者:
王玉珏郭凯陈驰吴少杰顾为望
关键词:
线粒体小肠辐射西藏小型猪
Keywords:
mitochondria intestines radiation Tibet minipigs
摘要:
目的观察不同剂量X线全身照射对西藏小型猪小肠线粒体的影响及其变化特点。方法将18头成年,健康,雄性西藏小
型猪分为6组。实验组5组,对照组1组(n=3头/组)。分别接受2,5,8,11,14Gy全身X线照射。照射后72h麻醉处死并采集小
肠组织分别检测线粒体相关基因的表达,线粒体呼吸链复合酶Ⅰ,Ⅱ,Ⅲ和Ⅳ活性,线粒体琥珀酸氧化呼吸链和NADH氧化呼吸
链的功能及观察线粒体的超微结构。结果8Gy以下剂量组的线粒体蛋白编码相关基因表达、呼吸链复合物活性及呼吸功能明
显低于对照组且随放射剂量的增加而降低;而8Gy以上剂量组,上述指标虽仍明显低于对照组,但不再随放射剂量的增加而继
续降低并维持于一较低水平。电镜下线粒体超微结构在2,5Gy时的改变主要表现为线粒体的扩张及轻微的电子密度降
低。8Gy及其后的剂量组则观察到更为明显的电子密度降低及线粒体的空泡化,嵴与线粒体外膜的连接缺失等。结论电离辐
射导致的小肠线粒体损害在一定剂量范围内(8Gy以下)随放射剂量的增加而愈发明显;但接受更高强度的照射后,其损伤程度
并不会随放射剂量的增加而继续增加。
Abstract:
ObjectiveTo investigate the injuries of intestinal mitochondria induced by different doses of whole-body radiation
in Tibet minipigs. MethodsEighteen Tibet minipigs were randomized into5radiation groups (n=3) and a control group (n=3).
The minipigs in the radiation groups were subject to a total body X-ray radiation at2, 5, 8, 11, or 14Gy, and72h after the
exposure, the mRNA expressions of the intestinal mitochondrial genes were examined using RT-PCR. The changes in the
respiratory chain complexes I-IV and the respiratory functions of succinate and NADH were assayed, and the intestinal
ultrastructures were observed using transmission electron microscopy (TEM) following the exposures.ResultsCompared with
those in the control group, the expression levels of the related mitochondrial genes, the activities of the respiratory chain
complexes and the function of the respiratory chain were significantly lowered in the radiation groups. At the doses
below8Gy, the exposures caused significant reduction in the measurements as the radiation doses increased, but at higher
doses, these measurements showed no further reductions. Ultrastructurally, exposures at2and5Gy caused mitochondrial
expansion and mild reduction of the density, whereas radiation at8Gy or greater resulted in vacuolar changes and obvious
expansion of the mitochondria with damages of the mitochondrial cristae and membranes.ConclusionBelow the doses of8
Gy, intestinal mitochondrial damages in the minipigs increase with the radiation dose, but at higher doses, the damages do not
further increase with the radiation dose.

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更新日期/Last Update: 1900-01-01