[1]梁卫江,张万岱,陈村龙,等.TGFα对胃癌细胞cyclin E和cyclin D1表达的影响[J].南方医科大学学报,2006,(08):1255-1257.
 LIANG Wei-jiang,ZHANG Wan-dai,CHEN Cun-long,et al.Effect of transforming growth factor α on the expression of cyclin E and cyclin D1 in gastric carcinoma cells[J].Journal of Southern Medical University,2006,(08):1255-1257.
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TGFα对胃癌细胞cyclin E和cyclin D1表达的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2006年08期
页码:
1255-1257
栏目:
论著·基础研究
出版日期:
2000-01-01

文章信息/Info

Title:
Effect of transforming growth factor α on the expression of cyclin E and cyclin D1 in gastric carcinoma cells
作者:
梁卫江; 张万岱; 陈村龙; 姚永莉; 罗荣城;
南方医科大学南方医院肿瘤中心; 南方医科大学南方医院消化内科; 南方医科大学南方医院肿瘤中心 广东广州510515; 广东广州510515;
Author(s):
LIANG Wei-jiang1 ZHANG Wan-dai2 CHEN Cun-long2 YAO Yong-li2 LUO Rong-cheng1 1Cancer Center 2Department of Digestive Disease Nanfang Hospital Southern Medical University Guangzhou 510515 China
关键词:
胃癌 TGFα cyclin E cyclin D1
Keywords:
gastric carcinoma transforming growth factor α cyclin E cyclin D1
分类号:
R735.2
摘要:
目的探讨TGFα对胃癌细胞cyclinE和D1表达的促进作用,为研究TGFα促胃癌发生发展机制提供重要信息。方法①细胞体外培养,用无血清RPMI-1640阻断法进行G0/G1期同步化,用碘化丙啶染色和流式细胞术,检测其同步化率;②细胞经G0/G1期同步化后,给予2.5%低浓度血清+RPMI-1640培养处理,以及在此基础上分别加入10、30、50μg/LTGFα处理,均作用5h;用免疫荧光染色及流式细胞术,检测细胞cyclinE和D1的表达情况。结果细胞经无血清RPMI-1640阻断法处理,其G0/G1期的百分率由常规培养的54%上升到72%;加入TGFα处理后,细胞cyclinE和D1表达均显著高于单用低血清培养(均P<0.001),并随TGFα剂量的增加而增加;当TGFα浓度为50μg/L时,两者的表达与单用低血清培养比,分别增加了25.18%和27.52%(均P<0.001)。结论TGFα可显著促进胃癌细胞cyclinE和D1的表达,从而发挥其促细胞周期进程的作用。
Abstract:
Objective To explore the effect of transforming growth factor α (TGFα) on the expression of cyclin E and D1 in gastric carcinoma cells. Methods Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G0/G1 phase in serum-free RPMI-1640. The percentage of the cells at G0/G1 phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 μg/L TGFα for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM. Results The percentage of the cells at G0/G1 phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFα treatment of the cells synchronized at G0/G1 phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFα of 50 μg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001). Conclusion TGFα can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.

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更新日期/Last Update: 1900-01-01