[1]尹瑞雪,陆兵勋,王立新,等.缺血再灌注损伤大鼠脑内神经巢蛋白的变化及通心络对它的影响[J].南方医科大学学报,2006,(06):777-779.
 YIN Rui-xue,LU Bing-xun,WANG Li-xin,et al.Nestin activation after rat cerebral ischemia-reperfusion injury and its changes in response to Tongxinluo treatment[J].Journal of Southern Medical University,2006,(06):777-779.
点击复制

缺血再灌注损伤大鼠脑内神经巢蛋白的变化及通心络对它的影响()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2006年06期
页码:
777-779
栏目:
论著·基础研究
出版日期:
2000-01-01

文章信息/Info

Title:
Nestin activation after rat cerebral ischemia-reperfusion injury and its changes in response to Tongxinluo treatment
作者:
尹瑞雪; 陆兵勋; 王立新; 范建中; 卢昌均; 刘忆星;
南方医科大学南方医院神经内科; 南方医科大学南方医院神经内科 广东广州510515; 广东广州510515;
Author(s):
YIN Rui-xue LU Bing-xun WANG Li-xin FAN Jian-zhong LU Chang-jun LIU Yi-xing Department of Neurology Nanfang Hospital Southern Medical University Guangzhou 510515 China
关键词:
脑缺血 神经巢蛋白 通心络 大鼠
Keywords:
cerebral ischemia nestin Tongxinluo rats
分类号:
R285.5
摘要:
目的探讨神经巢蛋白(nestin)在脑缺血再灌注损伤后神经细胞的活化增殖情况及通心络对其影响。方法采用大鼠缺血再灌注损伤(MCAO)模型,应用免疫组织化学方法观察缺血后3、7、14以及21d缺血侧室管膜及室管膜下区(SVZ)、海马齿状回(HDG)神经巢蛋白的变化。给予模型大鼠通心络灌胃,观察神经干细胞增殖分化的变化。结果神经巢蛋白阳性细胞随缺血再灌注时间的延长,荧光强度值增加,第7、14、21天组与假手术组比较,差异具有显著性(P<0.05)。造模后通心络组BrdU阳性细胞荧光强度值和BrdU+nestin免疫双标荧光强度值均高于脑缺血再灌注模型组,差异显著(P<0.05)。结论大鼠缺血再灌注损伤后可引起其缺血侧SVZ、HDG区神经干细胞反应和增殖;而通心络可显著增加MCAO大鼠神经干细胞增殖分化能力。?
Abstract:
Objective To investigate nestin activation in rat brain subjected to ischemia-reperfusion injury and its changes in response to Tongxinluo treatment. Methods Cerebral ischemia was induced by temporary middle cerebral artery occlusion (MCAO) in rats. At 3, 7, 14 and 21 days after MCAO, nestin expression in the ependyma, subventricular zone (SVZ), hippocampal subdentate gyrus zone (HDG) of the rats treated with Tongxinluo were guantified by immunohistochemistry. Results Compared with the sham operation group, nestin was significantly increased 7, 14 and 21 days after MCAO (P<0.05), and immunofluorescence of BrdU+nestin-positive neurons significantly increased in the SVZ. After treatment with Tongxinluo, the number of BrdU-positive neurons and BrdU+nestin-positive neurons significantly increased as compared with MCAO group (P<0.05). Conclusion Focal cerebral ischemia in the rat results in rapid response and proliferation of neural stem cells in the SVZ and HDG in the ischemic hemisphere, and Tongxinluo may enhance the differentiation and proliferation capacity of the neural stem cells after MCAO.?

相似文献/References:

[1]张兰兰,曹琼,胡子有,等.槲皮素对成年大鼠局灶性脑缺血后侧脑室室管膜下区神经干细胞增殖的影响[J].南方医科大学学报,2011,(07):1200.
[2]侯德仁,沙地克·沙吾提,刘建锋,等.意向运动疗法对大鼠局灶性脑缺血后GFAP和SYP表达的影响[J].南方医科大学学报,2011,(09):1543.
[3]王群,李永军,陆兵勋.甲状腺激素对慢性脑缺血大鼠认知功能障碍的改善作用[J].南方医科大学学报,2005,(01):106.
 WANG Qun,LI Yong-jun,LU Bing-xun.Effects of thyroid hormone on cognitive function in rats with chronic cerebral ischemia[J].Journal of Southern Medical University,2005,(06):106.
[4]廖春来,佟丽,陈育尧.补阳还五汤对脑缺血大鼠nNOS免疫阳性神经元的影响[J].南方医科大学学报,2004,(08):864.
 LIAO Chun-lai,0TONG Li,CHEN Yu-yao.Effect of Buyanghuanwu decoction on neuronal nitric oxide synthase expression after permanent focal cerebral ischemia in rats[J].Journal of Southern Medical University,2004,(06):864.
[5]朱心红,杨建明,高天明.脑心通对大鼠脑缺血海马CA1区神经元损伤的保护作用[J].南方医科大学学报,2004,(10):1123.
 ZHU Xin-hong,YANG Jian-ming,GAO Tian-ming.Neuroprotective effects of Naoxintong against neuronal injury in hippocampal CA1 region following transient forebrain ischemia in rats[J].Journal of Southern Medical University,2004,(06):1123.
[6]黄丽,王春,李言,等.针刺预处理对脑缺血大鼠小脑浦肯野细胞动作电位的影响[J].南方医科大学学报,2018,(06):677.

备注/Memo

备注/Memo:
国家自然科学基金(30400612)~~
更新日期/Last Update: 1900-01-01