[1]韩春,王爽,刘莉,等.鼻咽癌细胞肝转移亚株的建立[J].南方医科大学学报,2006,(05):654-657.
 HAN Chun,WANG Shuang,LIU Li,et al.Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line[J].Journal of Southern Medical University,2006,(05):654-657.
点击复制

鼻咽癌细胞肝转移亚株的建立()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2006年05期
页码:
654-657
栏目:
论著·基础研究
出版日期:
2000-01-01

文章信息/Info

Title:
Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line
作者:
韩春; 王爽; 刘莉; 姚开泰;
南方医科大学病理教研室; 南方医科大学病理教研室 广东广州510515; 广东广州510515;
Author(s):
HAN ChunWANG ShuangLIU LiYAO Kai-tai Department of PathologySouthern Medical UniversityGuangzhou 510515China
关键词:
鼻咽癌 肝转移 肿瘤侵袭 细胞亚株
Keywords:
nasopharyngeal carcinoma liver metastasis invasion subline
分类号:
R739.63
摘要:
目的建立鼻咽癌细胞肝转移亚株,研究鼻咽癌肝转移机制。方法利用裸鼠体内传代的方法获得肝转移细胞亚株,并用体内和体外实验来观察其侵袭转移能力。结果经体内反复筛选得到肝转移能力高的亚株5-8F-H3B-EGFP,其侵袭性、运动性以及形成肝转移灶的能力均大于母系5-8F-EGFP,并具有较高的肝脏亲和性。结论该亚株的建立为今后研究鼻咽癌肝转移的分子机制奠定了基础。?
Abstract:
Objective To establish a hepatic metastatic subline of nasopharyngeal carcinoma(NPC)cell line.Methods NPC cells metastatic to the liver were isolated from nude mice and the invasion and metastatic ability of the cells was observed in vivo and in vitro.Results and Conclusion The invasion and metastasis activity of 5-8F-H3B-EGFP(an in vivo isolate with enhanced liver metastatic behaviors)were enhanced obviously in comparison with the parent cell line 5-8F-EGFP.This subline may be useful for cloning genes related to liver metastasis of NPC.

相似文献/References:

[1]陈智贤,孙爱民,陈勇,等.放射敏感性、X线辐射剂量对鼻咽癌细胞miR-7表达的影响[J].南方医科大学学报,2010,(08):1810.
 CHEN Zhi-xian,SUN Ai-min,CHEN Yong,et al.Effects of radiosensitivity and X-ray dose on miR-7 expression in nasopharyngeal carcinoma[J].Journal of Southern Medical University,2010,(05):1810.
[2]熊艺颖,黄仲曦.来自不同地区的两组鼻咽癌活检组织样本共同失调的基因功能及通路[J].南方医科大学学报,2011,(03):414.
[3]刘晓东,季明芳.p53通路相关基因和EZH2在鼻咽癌中的表达关系及意义[J].南方医科大学学报,2011,(06):1029.
[4]江庆萍,刘少颜,何秀芳,等.鼻咽癌中MAP3K5和Epstein-Barr病毒编码的miR-BART22表达的关系及意义[J].南方医科大学学报,2011,(07):1146.
[5]柏志全,李华荣,张海峰,等.氯通道在藤黄酸诱导低分化鼻咽癌细胞凋亡中的作用[J].南方医科大学学报,2011,(08):1304.
[6]陈静平,龙军,张宏征.EB病毒壳抗原抗体定性检测在鼻咽癌筛查中的作用[J].南方医科大学学报,2011,(09):1637.
[7]王宏梅,石玉生,李启生,等.毛细管扩张症突变基因启动子区单核苷酸多态性位点rs189037与广东鼻咽癌相关性研究[J].南方医科大学学报,2011,(11):1863.
[8]李贵平,黄凯,张辉,等.188Re-Herceptin放免导向治疗鼻咽癌裸鼠模型的实验研究[J].南方医科大学学报,2006,(04):459.
 LI Gui-ping,HUANG Kai,ZHANG Hui Departmant of Nuclear Medicine,et al.Efficacy of 188Re-Herceptin radioimmunotherapy in nude mouse model of nasopharyngeal carcinoma[J].Journal of Southern Medical University,2006,(05):459.
[9]王剑,吴德华,陈龙华.铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖[J].南方医科大学学报,2012,(04):544.
[10]张三泉,彭宏,将会勇,等.人KIAA1173基因的克隆、转染及生物学特性的实验研究[J].南方医科大学学报,2005,(10):1216.
 ZHANG San-quan,PENG Hong,JIANG Hui-yong,et al.Cloning of human KIAA1173 gene and biological characterization of transfected 6-10B cells[J].Journal of Southern Medical University,2005,(05):1216.

更新日期/Last Update: 1900-01-01