[1]胡孝渠,秦利,苏逢锡,等.载体介导HER-2RNA干扰治疗乳腺癌研究[J].南方医科大学学报,2006,(05):570-572.
 HU Xiao-qu,QIN Li,SU Feng-xi,et al.Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer[J].Journal of Southern Medical University,2006,(05):570-572.
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载体介导HER-2RNA干扰治疗乳腺癌研究()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2006年05期
页码:
570-572
栏目:
论著·基础研究
出版日期:
2000-01-01

文章信息/Info

Title:
Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer
作者:
胡孝渠; 秦利; 苏逢锡; 姚和瑞; 陈积圣; 龚畅; 郭巨江; 于风燕; 贾海霞;
中山大学第二医院乳腺肿瘤中心; 中山大学第二医院肿瘤内科; 中山大学第二医院乳腺肿瘤中心 广东广州510272; 广东广州510272;
Author(s):
HU Xiao-qu1QIN Li1SU Feng-xi1YAO He-rui2CHEN Ji-sheng1GONG Chang1GUO Ju-jiang1YU Feng-yan1JIA Hai-xia1 1Center of Breast Cancer2Department of OncologySecond Hospital of Sun Yat-sen UniversityGuangzhou 510272China
关键词:
乳腺癌 RNA干扰 质粒载体
Keywords:
breast cancer plasmid vector RNA interference
分类号:
R737.9
摘要:
目的探讨载体介导HER-2RNA干扰治疗HER-2阳性乳腺癌的可行性。方法构建能够产生HER-2siRNA的质粒载体,利用载体转染HER-2阳性乳腺癌细胞SKBR-3,利用RT-PCR与Westernblot检测HER-2mRNA与蛋白表达情况,同时观察转染后SKBR-3细胞凋亡及生长情况。结果SKBR-3细胞HER-2受到载体介导的RNA干扰后,HER-2mRNA及蛋白表达出现明显下调,细胞凋亡增加,肿瘤细胞生长受到抑制。结论乳腺癌HER-2是理想的RNA干扰治疗靶点,靶向HER-2的RNA干扰可以诱导肿瘤凋亡,抑制肿瘤生长。
Abstract:
Objective To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.Methods A plasmid vector capable of mediating HER-2 RNA interference was constructed,and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector.The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting,and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.Results The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference,which also resulted in tumor cell growth inhibition and increased number apoptotic cells.Conclusion HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.

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备注/Memo

备注/Memo:
国家自然科学基金(30440021);国家杰出青年自然科学基金(30525022)~~
更新日期/Last Update: 1900-01-01