[1]李忠海,刘尚喜,侯凡凡,等.晚期氧化蛋白产物对小鼠腹腔巨噬细胞产生一氧化氮的影响[J].南方医科大学学报,2006,(05):558-560.
 LI Zhong-hai,LIU SHANG-Xi,HOU Fan-fan,et al.Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophages[J].,2006,(05):558-560.
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晚期氧化蛋白产物对小鼠腹腔巨噬细胞产生一氧化氮的影响()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2006年05期
页码:
558-560
栏目:
论著·基础研究
出版日期:
2000-01-01

文章信息/Info

Title:
Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophages
作者:
李忠海; 刘尚喜; 侯凡凡; 王燕群;
南方医科大学南方医院肾内科; 南方医科大学南方医院肾内科 广东广州510515; 广东广州510515;
Author(s):
LI Zhong-haiLIU SHANG-XiHOU Fan-fanWANG Yan-qun Department of NephrologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515China
关键词:
晚期氧化蛋白产物 氧化应激 巨噬细胞 一氧化氮
Keywords:
advanced oxidation protein products oxidative stress macrophages nitric oxide
分类号:
R363
摘要:
目的研究晚期氧化蛋白产物(AOPP)对小鼠腹腔巨噬细胞(MPM)产生一氧化氮的影响。方法用次氯酸氧化牛血清白蛋白(BSA)制备的AOPP-BSA单独或与内毒素(LPS)一起与MPM共同培养或用AOPP-BSA预处理后再用LPS刺激MPM,用Griess试剂法测定培养上清一氧化氮的含量;MTT法测定细胞活力。结果AOPP修饰可使BSA诱导MPM产生一氧化氮的量显著性降低。同时,AOPP-BSA预处理或与LPS一起共同刺激巨噬细胞均可显著性抑制LPS诱导的MPM产生一氧化氮,抑制程度与BSA的氧化修饰程度AOPP-BSA的浓度有关。结论晚期氧化蛋白产物可抑制MPM诱导型一氧化氮的产生。?
Abstract:
Objective To investigate the effect of advanced oxidation protein products(AOPP)on nitric oxide(NO)production in mouse peritoneal macrophages(MPMs).Methods MPMs were incubated in the absence or presence of lipopolysaccharide(LPS)with AOPP-modified bovine serum albumin(BSA)prepared by exposure of BSA to hypoclorous acid or pre-treated with AOPP-BSA and subsequent stimulation with LPS.NO production in the supernatants of the culture media was determined spectrophotometrically using Griess method.The cell viability was measured by MTT assay.Results BSA induced significant NO production in MPMs.AOPP modification of BSA significantly inhibited NO production,and AOPP-BSA exhibited time-and dose-dependent inhibition of NO production induced by LPS in MPMs incubated together with LPS or pre-treated before LPS stimulation.Conclusion AOPP-BSA is capable of inhibiting inducible NO production in MPMs.

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备注/Memo

备注/Memo:
国家自然科学基金重点项目(30330300);面上项目(30370370)~~
更新日期/Last Update: 1900-01-01