[1]姚红兵,吴爱国,陈佑江,等.运用组织芯片技术检测COX-2在结直肠癌中的表达及其临床意义[J].南方医科大学学报,2005,(12):1524-1528.
 YAO Hong-bing,WU Ai-guo,CHEN You-jiang,et al.Expression of COX-2 protein in colorectal carcinoma and the clinical implication[J].,2005,(12):1524-1528.
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运用组织芯片技术检测COX-2在结直肠癌中的表达及其临床意义()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年12期
页码:
1524-1528
栏目:
出版日期:
2005-12-01

文章信息/Info

Title:
Expression of COX-2 protein in colorectal carcinoma and the clinical implication
作者:
姚红兵1 吴爱国2 陈佑江1 唐斌华1
1. 解放军第181中心医院普通外科, 广西, 桂林, 541002;
2. 南方医科大学珠江医院普通外科, 广东, 广州, 510282
Author(s):
YAO Hong-bing1 WU Ai-guo2 CHEN You-jiang1 TANG Bin-hua1
1. Department of General Surgery, 181 Central Hospital of PLA, Guilin 541002, China;
2. Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
关键词:
组织芯片COX-2结直肠癌预后
Keywords:
COX-2tissue microarraycolorectal carcinomaprognosis
分类号:
Q78;R735.3
摘要:
目的 探讨结直肠癌组织中COX-2的表达及其与各临床病理因素的关系,评价COX-2在结直肠癌预后判断中的价值。方法 应用组织芯片技术结合免疫组织化学SABC法,检测126例早中期结直肠癌组织中COX-2的表达情况,回顾性分析COX-2与各临床病理因素及预后之间的关系。结果 根据免疫组化染色强度,所有病例被分为COX-2高表达组和低表达组,其中高表达组有32例(25.4%),低表达组有94例(74.6%)。COX-2在结直肠癌中表达情况与年龄、性别、肿瘤大小、肿瘤部位、组织学类型、浸润深度、淋巴是否转移、Dukes分期均无相关。然而,高表达的COX-2与肿瘤复发、特别是血行转移显著相关(P<0.05)。两组之间生存率具有显著差异(P=0.0067),COX-2高表达组术后五年生存率显著低于低表达组。多因素回归模型分析结果显示,在潜在的预后因素中(年龄、性别、肿瘤大小、肿瘤部位、组织学类型、淋巴是否转移、Dukes分期、COX-2表达),COX-2表达和Dukes分期可作为是结直肠癌根治术后独立预后因素,COX-2的检测可作为结直肠癌患者预后判断的一个有价值的指标。结论 高表达的COX-2与肿瘤复发、特别是血行转移显著相关,COX-2的检测可作为结直肠癌患者预后判断的一个有价值的指标。应用组织芯片高效检测临床组织样本具有快速、方便、经济、准确的优点。
Abstract:
Objective To investigate the relationship between COX-2 expression and the clinicopathological factors in colorectal carcinoma and assess the prognostic value of COX-2 detection. Methods Tissue microarray and immunohistochemistry by SABC method was employed for detecting COX-2 expression in 126 patients with advanced colorectal cancer, and the relationship of COX-2 expression with the clinicopathological features and prognosis of the patients was retrospectively analyzed. Results The patients were divided into two groups of low and high COX-2 groups according to the grade and extent of COX-2 expression. High COX-2 expression was detected in 32 (25.4%) cases, and low expression in 94 (74.6%) cases. No significant correlation was noted between COX-2 expression and the patients’ age, sex, tumor size, tumor location, histological type, lymphatic-node metastasis and Dukes’ classification, but high COX-2 expression was strongly correlated with tumor recurrence and especially with blood-borne metastasis (P<0.05). The survival rate without tumor recurrence for high-and low-COX-2 groups was assessed by the Kaplan-Meier method and compared by log-rank test, which revealed significant difference between the two groups (P=0.0067). Multivariate analysis for all patients suggested that among the 8 prognostic factors (age, sex, tumor size, tumor location, histological type, lymphatic-node metastasis, Dukes’ stage, and COX-2 expression), Dukes’ stage and COX-2 expression was the independent significant factor related to disease-free survival. Conclusion The expression of COX-2 is strongly correlated with recurrence of colorectal cancer, especially with blood-borne metastasis. COX-2 is an independent factor for prognostic evaluation of the patients, and tissue microarray allows rapid, convenient, economic and accurate COX-2 detection for large-scale application.

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备注/Memo

备注/Memo:
收稿日期:2005-6-13。
作者简介:姚红兵(1974- ),男,2004年毕业于第一军医大学,临床医学硕士,医师,E-mail:luckstar0503@163.com.
更新日期/Last Update: 1900-01-01