[1]陈小林,何小洪,张焰,等.慢性体外反搏对高胆固醇血症猪动脉内皮细胞的影响[J].南方医科大学学报,2005,(12):1491-1493.
 CHEN Xiao-lin,HE Xiao-hong,ZHANG Yan,et al.Effect of chronic enhanced external counterpulsation on arterial endothelial cells of porcine with hypercholesteremia[J].Journal of Southern Medical University,2005,(12):1491-1493.
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慢性体外反搏对高胆固醇血症猪动脉内皮细胞的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年12期
页码:
1491-1493
栏目:
出版日期:
2005-12-01

文章信息/Info

Title:
Effect of chronic enhanced external counterpulsation on arterial endothelial cells of porcine with hypercholesteremia
作者:
陈小林 何小洪 张焰 钱月桃 梁陆光 方典秋 詹澄扬 郑振声 马虹
中山大学附属第一医院心内科, 广东, 广州, 510089
Author(s):
CHEN Xiao-lin HE Xiao-hong ZHANG Yan QIAN Yue-tao LIANG Lu-guang FANG Dian-qiu ZHAN Cheng-yang ZHENG Zhen-sheng MA Hong
Department of Cardiology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510089, China
关键词:
增强体外反搏高胆固醇血症内皮细胞蛋白质组学形态学
Keywords:
enhanced external counterpulsationhypercholesteremiaendothelial cellsproteomicsmorphology
分类号:
R54
摘要:
目的 探讨慢性增强型体外反博(EECP)能否在体修复高胆固醇血症引起的猪动脉内皮细胞(ECs)损伤。方法 高胆固醇血症猪反搏36h后,取前降支进行扫描电镜检查,收集胸主动脉ECs进行蛋白质组学检查。结果 高脂组冠脉ECs排列不规则、大量脱落、斑块形成,反搏组ECs呈流线型排列、脱落程度显著减轻;与高脂组相比,在反搏组的ECs检测到6种高表达的蛋白。结论 慢性EECP可能通过调节ECs蛋白表达,改善ECs粘附性和代谢及减轻ECs内皮凋亡,从而修复高胆固醇血症对动脉内皮形态和功能的损伤。
Abstract:
Objective To verify whether chronic enhanced external counterpulsation (EECP) may repair artery endothelial cells (ECs) damage resulted from hypercholesteremia in pigs. Methods EECP was performed for 36 hours in pigs with hypercholesteremia and the left descending artery (LDA) was isolated for scanning electron microscopy (SEM). The ECs were collected from the thoracic aorta and analyzed by proteomic technique. Results The ECs of hypercholesteremia pigs showed irregular arrangement with obvious desquamation of coronary ECs and formation of atherosclerotic plaque, and these lesions were milder in EECP-treated pigs. Six over-expressed proteins were detected in the endothelial cells in EECP-treated pigs in comparison with those of the hypercholesteremia pigs. Conclusion Chronic EECP helps restore cell morphology and repair functional damage of ECs resulted from hypercholesteremia by regulating endothelial protein expressions, and consequently improves cell adhesion and intracellular metabolism and reducesg EC apoptosis.

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备注/Memo

备注/Memo:
收稿日期:2005-4-6。
基金项目:国家“十五”科技攻关项目(2001BA706B07);国家自然科学基金(30127001)
作者简介:陈小林(1968- ),男,医学博士,现在广东省江门市人民医院/江门市心血管病研究所工作,主要从事冠心病的基础与临床研究,电话:020-87330775-815,E-mail:cxlmce68@yahoo.com.cn.
更新日期/Last Update: 1900-01-01