[1]芮勇宇,阚飙,高守一,等.霍乱弧菌JS94484株CTXEVCφ和nct-CTXnewφ基因组变异区序列分析[J].南方医科大学学报,2005,(11):1361-1364.
 RUI Yong-yu,KAN Biao,GAO Shou-yi,et al.Sequence analysis of the mutated genes in CTXEVCφ and nct-CTXnewφ genomes in Vibrio cholerae JS94484 strain[J].Journal of Southern Medical University,2005,(11):1361-1364.
点击复制

霍乱弧菌JS94484株CTXEVCφ和nct-CTXnewφ基因组变异区序列分析()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年11期
页码:
1361-1364
栏目:
出版日期:
2005-11-01

文章信息/Info

Title:
Sequence analysis of the mutated genes in CTXEVCφ and nct-CTXnewφ genomes in Vibrio cholerae JS94484 strain
作者:
芮勇宇1 阚飙2 高守一2 刘延清2 祁国明2
1. 南方医科大学南方医院检验科, 广东, 广州, 510515;
2. 中国疾病预防控制中心传染病预防控制所, 北京, 102206
Author(s):
RUI Yong-yu1 KAN Biao2 GAO Shou-yi2 LIU Yan-qing2 QI Guo-ming2
1. Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;
2. Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
关键词:
霍乱弧菌噬菌体毒力协同调节菌毛A亚单位聚合酶链反应序列分析
Keywords:
Vibrio choleraephagetoxin-coregulated pilus A subunit gene (tcpA)polymerase chain reaction (PCR)sequence analysis
分类号:
R379
摘要:
目的 对霍乱弧菌JS94484株CTXEVCφ和nct-CTXnewφ基因组变异区进行序列分析。方法 采用聚合酶链反应、基因测序及序列分析。结果 JS94484株CTXEVCφ基因组的ig1、rstR、ig2和ctxAB基因与EVC标准株N16961的高度同源。JS94484株nct-CTXnewφ基因组的ig1、rstR和ig2基因,与目前发现的3种类型的同源性均较低,趋异性均较高。CTXEVCφ和nct-CTXnewφ基因组zot基因末端约60bp的基因序列差别较大,推测的氨基酸序列差别也较大。JS94484株的tcpA基因序列与EVC标准株N16961的完全一致。结论 霍乱弧菌nct-CTXnewφ基因组的ig1、rstR和ig2基因为新类型,基因功能有待于进一步研究。
Abstract:
Objective To analyze the sequences of the mutated genes in CTXEVCφ and nct-CTXnewφ genomes in Vibrio cholerae JS94484 strain. Methods The mutated genes in CTXEVCφ and nct-CTXnewφ genome were obtained by PCR, sequenced and analyzed. Results ig1, rstR, ig2, and ctxAB genes in CTXEVCφ genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTXnewφ genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTXEVCφ and nct-CTXnewφ genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961. Conclusion ig1, rstR and ig2 genes of nct-CTXnewφ genome are of a novel type, and their functions await further investigation.

参考文献/References:

[1] Faruque SM, Albert ML, Mekalanos JJ. Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae[J]. Mol Biol Rev, 1998, 62(4):1301-14.
[2] Miller JF. Bacteriophage and the evolution of epidemic cholera[J].Infect Immun, 2003, 71(6): 1981-2.
[3] Faruque SM, Sack DA, Sack RB, et al. Emergence and evolution of Vibrio cholerae O139[J]. Proc Natl Acad Sci USA, 2003, 100(3):1301-9.
[4] Davis BM, Waldor MK. Filamentous phages linked to virulence of Vibrio cholerae[J]. Curr Opin Microbiol, 2003, 6(1): 35-42.
[5] Waldor MK, Mekalanos JJ. Lysogenic conversion by a filamentous phage encoding cholera toxin[J]. Science, 1996, 272:1910-4.
[6] Li M, Kotetishvili M, Chen Y, et al. Comparative genomic analyses of the vibrio pathogenicity island and cholera toxin prophage regions in nonepidemic serogroup strains of Vibrio cholerae[J]. Appl Environ Microbiol, 2003, 69(3): 1728-38.
[7] Kimsey HH, Waldor MK. The CTXphi repressor RstR binds DNA cooperatively to form tetrameric repressor-operator complexes[J]. J Biol Chem, 2004, 279(4): 2640-7.
[8] Davis BM, Kimsey HH, Chang W, et al. The Vibrio cholerae O139Calcutta bacteriophage CTXψ is infectious and encodes a novel repressor[J]. J Bacteriol, 1999, 181(21): 6779-87.
[9] 梁未丽,阚飙,高守一.编码霍乱毒素的霍乱弧菌溶原性噬菌体CTXψ研究概况[J].中华微生物学和免疫学杂志(Chin JMicrobiol Immunol),2003,23(8):657-62.
[10] 芮勇宇,阚飙,高守一,等.霍乱弧菌CTXψ基因组抑制子基因多态性分析[J].中国预防医学杂志(Chin Prev Med J),2003,4(霍乱增刊):211-3.
[11] Boyd EF, Heilpern A J, and Waldor MK. Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTX(phi)s by toxigenic Vibrio cholerae[J]. J Bacteriol,2000, 182(19): 5530-8.
[12] Karaolis DK, Lan R, Kaper JB, et al. Comparison of Vibrio cholerae pathogenicity islands in sixth and seventh pandemic strains[J]. Infect Immun, 2001, 69(3): 1947-52.

相似文献/References:

[1]芮勇宇,阚飙,高守一,等.不同群型霍乱弧菌recA、dnaE和mdh管家基因序列分析[J].南方医科大学学报,2006,(12):1720.
 RUI Yong-yu,KAN Biao,GAO Shou-yi,et al.Sequence analysis of housekeeping genes recA,dnaE,and mdh in different serogroups or biotypes of Vibrio cholerae isolated from China[J].Journal of Southern Medical University,2006,(11):1720.

备注/Memo

备注/Memo:
收稿日期:2005-4-7。
基金项目:国家自然科学基金(30470099;30170821)
作者简介:芮勇宇(1970-),男,副研究员,博士后,硕士导师,电话:020-61642145,E-mail:yongyurui@tom.com
更新日期/Last Update: 1900-01-01