[1]刘国炳,刘欣,庞战军,等.宫颈癌患者盆腔淋巴结HPV-DNA检测的临床意义[J].南方医科大学学报,2005,(10):1236-1239.
 LIU Guo-bing,LIU Xin,PANG Zhan-jun,et al.Significance of pelvic lymph node human papillomavirus DNA detection in cervical cancer[J].Journal of Southern Medical University,2005,(10):1236-1239.
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宫颈癌患者盆腔淋巴结HPV-DNA检测的临床意义()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年10期
页码:
1236-1239
栏目:
出版日期:
2005-10-01

文章信息/Info

Title:
Significance of pelvic lymph node human papillomavirus DNA detection in cervical cancer
作者:
刘国炳 刘欣 庞战军 邢福祺
南方医科大学南方医院妇产科, 广东, 广州, 510515
Author(s):
LIU Guo-bing LIU Xin PANG Zhan-jun XING Fu-qi
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
关键词:
人类乳头瘤病毒DNA聚合酶链反应淋巴转移宫颈肿瘤
Keywords:
human papillomavirusDNApolymerase chain reactionlymph node metastasescervical neoplasms
分类号:
R737.33
摘要:
目的 探讨宫颈癌患者盆腔淋巴结人类乳头瘤病毒(HPV)-DNA检测的临床意义。方法 采用GP-PCR方法扩增宫颈癌患者盆腔淋巴结组织HPV-DNAL1区基因序列,通过序列分析对HPV进行型别鉴定,分析淋巴结HPV阳性率与淋巴转移组织病理学结果之间的关系。结果 40例宫颈癌患者盆腔淋巴结HPV-DNA的检出率为40%;组织病理学发现淋巴转移的10例患者淋巴结HPV-DNA检测均为阳性;宫颈癌病灶中检测到HPV18型的患者盆腔淋巴结也都可检测到HPV18型。结论 盆腔淋巴结中检出HPV-DNA阳性早于组织病理学出现转移,因此检测盆腔淋巴结中HPV-DNA对早期的淋巴结转移具有提示作用,有助于发现组织病理学不易发现的淋巴结隐匿性转移,提示预后不良。宫颈检测到HPV18型的患者更易发生淋巴转移,是预后不良的一种高危类型。
Abstract:
Objective To assess the significance of human papillomavirus (HPV) DNA detection in the pelvic lymph nodes in cervical cancer. Methods HPV L1 gene fragment was amplified by HPV-specific PCR with general consensus primers from cervical cancer tissues. The types of HPV were identified by sequencing of the PCR product. The relationship between HPV positivity and pathological findings in metastatic pelvic lymph nodes of cervical cancer was analyzed. Results The positivity rate of HPV DNA was 40% in the pelvic lymph nodes in 40 cases of cervical cancer. HPV DNA was detected in the pelvic lymphatic nodes of all the 10 cases with pathologically confirmed lymph node metastasis of cervical cancer. HPV18 was detected in both of cervical and pelvic lymph nodes in some cases. Conclusions HPV DNA can be detected in the pelvic lymph nodes before pathological identification of lymph node metastasis and indicates early pelvic lymph node metastasis of cervical cancer. The presence of HPV18 often indicates high likeliness of lymph node metastasis and suggests poor prognosis of the patients.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2005-5-30。
基金项目:南方医院2002年新技术新业务课题
作者简介:刘国炳(1960- ),男,副教授、副主任医师,电话:020-61641888-87290,E-mail:lgb@fimmu.com
更新日期/Last Update: 1900-01-01