[1]余元龙,季明芳,何洁冰,等.紫杉醇、5-氟脲嘧啶抑制肝癌细胞BEL-7402生长和诱导凋亡的比较[J].南方医科大学学报,2005,(07):864-867.
 YU Yuan-long,JI Ming-fang,HE Jie-bing,et al.Effects of paclitaxel and 5-fluorouracil on growth inhibition and apoptosis of hepatoma cells:a comparative study[J].Journal of Southern Medical University,2005,(07):864-867.
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紫杉醇、5-氟脲嘧啶抑制肝癌细胞BEL-7402生长和诱导凋亡的比较()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年07期
页码:
864-867
栏目:
出版日期:
2005-07-01

文章信息/Info

Title:
Effects of paclitaxel and 5-fluorouracil on growth inhibition and apoptosis of hepatoma cells:a comparative study
作者:
余元龙1 季明芳2 何洁冰2 李晓玲2
1. 中山市人民医院普外科, 广东, 中山, 528403;
2. 中山市肿瘤研究所, 广东, 中山, 528400
Author(s):
YU Yuan-long1 JI Ming-fang2 HE Jie-bing2 LI Xiao-ling2
1. Department of General Surgery, Zhongshan People’s Hospital, Zhongshan 528403, China;
2. Cancer Research Institute of Zhongshan, Zhongshan 528400, China
关键词:
肝细胞癌紫杉醇5-氟脲嘧啶细胞凋亡
Keywords:
hepatoma cellspaclitaxel5-fluorouracilapoptosis
分类号:
R-33;R735.7
摘要:
目的 研究紫杉醇、5-氟脲嘧啶(5-Fu)抑制肝癌细胞BEL-7402增殖和诱导凋亡的效果。方法 采用ATP生物发光法检测肝癌细胞株的药物敏感性,以流式细胞术、形态学方法分析细胞凋亡和细胞周期。结果 BEL-7402对紫杉醇高度敏感,对5-Fu低度敏感,抑制作用均存在剂量、时间效应。紫杉醇组3种浓度诱导细胞凋亡率均明显高于无药对照组(P<0.05),但低于5-Fu组(P<0.01);紫杉醇组细胞死亡率显著高于5-Fu组(P<0.05)。紫杉醇诱导凋亡率随药物浓度降低而增加,而5-Fu诱导凋亡率随药物浓度降低而降低。结论 紫杉醇可抑制BEL-7402肝癌细胞增殖和诱导凋亡。5-Fu抑癌以诱导细胞凋亡为主,紫杉醇则以引起细胞坏死为主。
Abstract:
Objective To investigate the effect of paclitaxel and 5-flurouracil (5-Fu) on growth inhibition and apoptosis of human hepatoma BEL-7402 cells. Methods Growth inhibition of BEL-7402 cells treated with paclitaxel and 5-Fu,respectively,was measured by ATP-tumor chemosensitivity assay (ATP-TCA),and the cell cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination. Results BEL-7402 cells were highly sensitive to paclitaxel with growth inhibition observed in both dose- and time-dependent manners (IC50=5.58×10-7 mol/L).Paclitaxel induced significantly higher rate of cell apoptosis than the control group (P<0.05) but significantly lower rate than that induced by 5-Fu (P<0.01).Necrosis was observed predominantly in paclitaxel-treated cells whereas 5-Fu caused mainly cell apoptosis (P<0.05).Levels of apoptosis increased in proportion to the decrement of paclitaxel concentration but directly proportional to increment of 5-Fu concentration. Conclusions Paclitaxel and 5-Fu are effective in inducing growth inhibition and apoptosis of BEL-7402 cells.While 5-Fu causes mainly apoptosis in hepatoma cells,the anticancer mechanism of paclitaxel is predominantly through induction of necrosis.

参考文献/References:

[1] Wani MC, Taylor HL, Wall ME, et al. Plant antitumor agents. Ⅵ.The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia [J]. J Am Chem Soc, 1971, 93:2325-7.
[2] 余元龙,季明芳,李晓玲,等.体外药敏试验探讨肝脏灌注化疗的优化方案[J].中华肝脏病杂志,2004,12(9):558.
[3] 褚忠华,陈涛,区庆嘉,等.肿瘤体外药敏试验三磷酸腺苷生物发光法在肝癌化疗中的应用[J].中华实验外科杂志,2002,19(3):235-6.
[4] 何松,沈薇,沈鼎明.体外观察紫杉醇对肝癌HepG2细胞生长的影响[J].重庆医学,2003,32(3):268-70.
[5] 耿长新,曾昭冲,王吉耀.多烯紫杉醇抑制肝癌细胞生长及诱导凋亡[J].中华消化杂志,2003,23(6):381-2.
[6] Yeung TK, Germord C, Chen X, et al. Themode of action oftaxol:apoptosis at low concentration and necrosis ay high concentration[J]. Biochem Biophy Res Commun, 1999, 263: 398-404.
[7] 李瑛,石廷章,赵卫红.泰素帝诱导人肺腺癌细胞凋亡的实验研究[J].中华肿瘤杂志,2000,22(3):208-11.
[8] Arends MJ, Wyllie AH. Apoptosis: mechanisms and roles in pathology[J]. Int Rev Exp Pathol, 1991, 32: 223-54.
[9] Ronald S, Kathryn A. Lack of correlation between mitotic arrest or apoptosis and antitumor effect of docetaxel [J]. Cancer Chemother Pharmacol, 1999, 43: 165-72.
[10] Levin S, Bucci TJ, Cohen SM, et al. The nomenclature of cell death:recommendations of an ad hoc Committee of the Society of Toxicologic Pathologists [J]. Toxicol Pathol, 1999, 27(4): 484-90.

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备注/Memo

备注/Memo:
收稿日期:2005-2-22。
基金项目:广东省医学科研基金(A2002880)
作者简介:余元龙(1964- ),男,硕士,副主任医师
更新日期/Last Update: 1900-01-01