[1]周海宽,赵永斌,郑少斌,等.S100A4基因差异表达与肾癌细胞分化、转移的关系[J].南方医科大学学报,2005,(06):712-714,717.
 ZHOU Hai-kuan,ZHAO Yong-bin,ZHENG Shao-bin,et al.Association of S100A4 mRNA expression with differentiation and metastasis of renal cell carcinoma[J].,2005,(06):712-714,717.
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S100A4基因差异表达与肾癌细胞分化、转移的关系()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年06期
页码:
712-714,717
栏目:
出版日期:
2005-06-01

文章信息/Info

Title:
Association of S100A4 mRNA expression with differentiation and metastasis of renal cell carcinoma
作者:
周海宽1 赵永斌2 郑少斌1 陈彤1 毛向明1 齐桓1 吴容娟1
1. 南方医科大学南方医院泌尿外科, 广东, 广州, 510515;
2. 广州军区广州总医院泌尿外科, 广东, 广州, 510010
Author(s):
ZHOU Hai-kuan1 ZHAO Yong-bin2 ZHENG Shao-bin1 CHEN Tong1 MAO Xiang-ming1 QI Huan1 WU Rong-juan1
1. Department of Urological Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2. Department of Urological Surgery, General Hospital of Guangzhou Command, Guangzhou 510010, China
关键词:
肾肿瘤S100A4基因表达细胞分化肿瘤转移
Keywords:
kidney neoplasmsS100A4gene expressioncell differentiationneoplasm metastasis
分类号:
R394.2;R737.11
摘要:
目的 探讨S100A4基因mRNA表达与肾癌细胞分化、转移的关系。方法 提取31例肾癌及正常组织配对标本的总RNA,用RT-PCR方法测定S100A4基因表达,并结合临床资料,对S100A4基因差异表达与肾癌病人临床表现的相关性进行分析研究。结果 31例配对标本采用RT-PCR方法分别进行S100A4mRNA荧光检测比较,29例标本肿瘤组织中S100A4基因mRNA表达明显高于癌旁正常肾组织。其中低分化肿瘤组病人的S100A4指数高于高分化组(7.94vs5.06,P<0.001);发生转移(包括淋巴结转移和远处转移)者高于无转移者(9.61vs5.53,P<0.001);低年龄组病人(≤50岁)S100A4指数与高年龄组无明显差异(6.31vs6.66,P>0.05);肾颗粒细胞癌组与透明细胞癌组无明显差异(6.98vs6.02,P>0.05);肿瘤直径≤5cm组与>5cm组无明显差异(5.95vs6.93,P>0.05)。结论 肾癌组织中S100A4基因mRNA可望作为肾细胞癌的病情发展及指导临床治疗的标记物之一;S100A4基因表达与肾细胞癌分化有关,癌组织S100A4mRNA测定有助于判定肿瘤的预后;S100A4基因差异表达与肾细胞癌转移明显相关。
Abstract:
Objective To study the association of S100A4 mRNA expression level with the differentiation and metastasis of renal cell carcinoma (RCC). Methods Tissue specimens were obtained from 31 patients undergoing surgery for renal cell carcinomas between May 2001 and May 2004. Reverse transcriptional PCR was performed for amplification of S100A4 mRNA (212 bp) from 31 RCC tissues and matched normal kidney tissues besides the tumor, and the differential S100A4 mRNA expression was analyzed for its association with the clinical manifestations of the patients. Results S100A4 mRNA expression was detected in all the RCC tissues and normal tissues adjacent to the carcinoma using fluorescence method, and in 29 cases, S100A4 mRNA expression was significantly higher in the RCC tissues than in the matched adjacent tissues. One case had high expressions in both tissues. Patients with poorly differentiated RCC had significantly higher S100A4 expression index than those with well differentiated RCC (7.94 vs 5.06, P<0.001), and patients with metastasis also had higher expression index (9.61 vs 5.53, P<0.001). No obvious difference was noted in the expression index between granular cell tumor and clear cell carcinoma (6.98 vs 6.02, P>0.05). Conclusion S100A4 may serve as a marker for assessing the progression of RCC and provide assistance for clinical therapeutic decisions. S100A4 mRNA expression is correlated to RCC differentiation and may facilitate prognostic evaluation of RCC. Differential expression of S100A4 mRNA is correlated to the metastasis of RCC.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-9-25。
作者简介:周海宽(1963- ),男,博士,电话:020-61641799
更新日期/Last Update: 1900-01-01