[1]赵军,宗利丽,李亚里.整合素avβ3单克隆抗体对小鼠子宫内膜异位病灶的抑制作用[J].南方医科大学学报,2005,(06):709-711.
 ZHAO Jun,ZONG Li-li,LI Ya-li.Inhibitory effects of integrin avβ3 monoclonal antibody on implanted lesions of endometriosis model of SCID mouse[J].,2005,(06):709-711.
点击复制

整合素avβ3单克隆抗体对小鼠子宫内膜异位病灶的抑制作用()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年06期
页码:
709-711
栏目:
出版日期:
2005-06-01

文章信息/Info

Title:
Inhibitory effects of integrin avβ3 monoclonal antibody on implanted lesions of endometriosis model of SCID mouse
作者:
赵军1 宗利丽2 李亚里1
1. 解放军总医院妇产科, 北京, 100853;
2. 南方医科大学珠江医院妇产科, 广东, 广州, 510282
Author(s):
ZHAO Jun1 ZONG Li-li2 LI Ya-li1
1. Department of Obstetrics and Gynecology, General Hospital of PLA, Beijing 100853, China; 2. Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China
关键词:
子宫内膜异位症整合素avβ3SCID小鼠
Keywords:
endometriosisintegrin avβ3SCID mouse
分类号:
R711.71
摘要:
目的 研究整合素avβ3单克隆抗体对SCID小鼠子宫内膜异位病灶生长和微血管形成的影响。方法 建立人子宫内膜异位症小鼠皮下移植病灶模型。治疗组尾静脉注射整合素avβ3单克隆抗体Lm609(250μg/只),对照组给予相应体积的PBS,1周2次。4周后处死动物,测量病灶,采用免疫组化法检测病灶微血管密度(MVD)及整合素avβ3的表达。结果 治疗组与对照组皮下移植病灶质量分别为(0.82±0.09)g和(0.51±0.93)g(P<0.05),病灶组织中MVD则分别为31.2±4.4和14.4±1.8(P<0.05),治疗组病灶受到显著抑制。结论 整合素avβ3单克隆抗体通过抑制微血管形成而抑制子宫内膜异位病灶的生长。
Abstract:
Objective To explore the potential use of integrin avβ3 (Lm609) monoclonal antibody against ectopic lesion and microvessel angiogenesis of human endometriosis xenografts in SCID mice. Methods The endometrial tissues from endometriosis patients were inoculated subcutaneously into SCID mouse, which were treated with Lm609, an inhibitor of angiogenesis, twice a week, for 3 weeks. The mice were sacrificed and immunohistochemical staining was employed to determine the microvessel density (MVD) and expression of integrin avβ3 in the lesion tissues. Results Lm609 inhibited the growth of the ectopic lesion. The lesion weighted 0.82±0.09 g in the treated group versus 0.51±0.93 g in the control group (P<0.05). The MVDs of the treated and control groups were 31.2±4.4 and 14.4±1.8 respectively (P<0.05). Conclusion Lm609 can inhibit the growth of ectopic lesion by reducing angiogenesis in SCID mouse.

参考文献/References:

[1] Strathy JH, Molgaard CA, Coulam CB, et al. Endometriosis and infertility:a laparoscopic study of endometriosis among fertile and infertile women [J]. Fertil Steril, 1982, 38(6):667-72.
[2] Maas JWM, Le Noble FAC, Dunselman GAJ, et al. The chick embryo chorioallantoic membrane as a model to investigate the angiogenic properties of human endometrium [J]. Gynecol Obstet Invest, 1999, 48:108-12.
[3] Zamah NM, Dodson MG, Stephens LC, et al. Transplantation of normal and ectopic human endometrial tissue into athymic nude mice[J]. Am J Obstet Gynecol, 1984, 149:591-7.
[4] Blezinger P, Wang J. Systemic inhibition of tumor growth and tumor metastases by intramuscular administration of the endostatin gene[J]. Nat Biotechnol, 1999, 17(4):343-8.
[5] Breasalier RS, HO SB, Schoeppner HL, et al. Enhanced salvation of mucin-associated carbohydrate structures in human colon cancer metastasis [J]. Gastroenterology, 1996, 110(5):1354-67.
[6] Horton MA. The alpha v beta 3 integrin "vitronectin receptor" [J].Int J Biochem Cell Biol, 1997, 29721-5.
[7] Eliceiri BP, Cheresh DA. Role of alpha v integrins during angiogenesis[J]. Cancer J, 2000, 63:S245-9.
[8] Brooks PC, Stromblad S, Klemke R, et al. Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin[J]. J Clin Invest, 1995, 96:1815-22.
[9] Hii LL, Rogers PA. Endometrial vascular and glandular:expression of integrin alpha(v) beta3 in women with and without endometriosis[J]. Hum Reprod, 1998, 13(4):1030-5.
[10] Gutheil JC, Campbell TN, Pierce PR, et al. Targeted antiangiogenic therapy for cancer using Vitaxin:a humanized monoclonal antibody to the integrin alpha v beta3 [J]. Clin Cancer Res, 2000, 6 (8):3056-61.
[11] Sparano JA, Bernardo P, Stephenson P, et al. Randomized phase Ⅲtrial of marimastat versus placebo in patients with metastatic breast cancer who have responding or stable disease after first-line chemotherapy:Eastern Cooperative Oncology Group trial E2196[J]. J Clin Oncol, 2004, 22:4683-90.

相似文献/References:

[1]易华,卢月,陈剑坤,等. 青藤碱对子宫内膜异位症大鼠异位组织TNF-琢和NF-资B 水平的影响[J].南方医科大学学报,2010,(08):1874.
 YI Hua,LU Yue,CHEN Jian-kun,et al.Effect of sinomenine on tumor necrosis factor-琢and nuclear factor-资B in the heterotopictissue in rats with endometriosis[J].,2010,(06):1874.
[2]李洁,汪淑娟,孙璐,等.抑癌基因ARHI 在子宫内膜异位症及非子宫内膜异位症患者中差异表达的意义[J].南方医科大学学报,2011,(05):796.
[3]罗敏,何援利,张宏斌,等.广东妇女子宫内膜异位症与肿瘤坏死因子-β基因多态性的相关性研究[J].南方医科大学学报,2006,(08):1163.
 LUO Min,HE Yuan-li,ZHANG Hong-bin,et al.Association of tumor necrosis factors-β gene polymorphism with endometriosis in women in Guangdong Province[J].,2006,(06):1163.
[4]宗利丽,李亚里,宋三泰,等.子宫内膜异位症患者肝细胞生长因子及其受体c-Met的表达[J].南方医科大学学报,2004,(06):619.
 ZONG Li-li,LI Ya-li,SONG San-tai,et al.Expression of hepatocyte growth factor and its receptor c-met gene in the endometrium of women with endometriosis[J].,2004,(06):619.
[5]刘木彪,何援利,彭冬先.子宫内膜异位症患者腹腔液血管内皮生长因子与内抑素的表达[J].南方医科大学学报,2004,(01):69.
 LIU Mu-biao,HE Yuan-li,PENG Dong-xian.Expression of vascular endothelial growth factor and endostatin in peritoneal fluid of patients with endometriosis[J].,2004,(06):69.
[6]蔡珠华,何援利,彭冬先.子宫内膜异位症患者血清中ENA-78的表达[J].南方医科大学学报,2005,(04):464.
 CAI Zhu-hua,HE Yuan-li,PENG Dong-xian.Changes of serum epithelial neutrophil-activating peptide-78 in patients with endometriosis[J].,2005,(06):464.
[7]王芳,何援利,彭冬先,等.子宫内膜异位症患者核转录因子-κB及细胞间粘附分子-1的表达[J].南方医科大学学报,2005,(06):703.
 WANG Fang,HE Yuan-li,PENG Dong-xian,et al.Expressions of nuclear factor-kappaB and intercellular adhesion molecule-1 in endometriosis[J].,2005,(06):703.
[8]彭冬先,何援利,丘立文.慢病毒介导shRNA沉默survivin基因对鸡胚绒毛尿囊膜子宫内膜异位症模型的影响[J].南方医科大学学报,2012,(07):995.
[9]刘木彪,黄雪梅,许苏容,等.叶酸在预防胎源性子宫内膜异位症易感基因HOXA10异常甲基化中的作用[J].南方医科大学学报,2013,(06):926.
[10]侯震晖,全松,钱卫平,等.子宫内膜异位症患者体质量指数与体外受精—胚胎移植周期临床结局的关系[J].南方医科大学学报,2014,(03):379.

备注/Memo

备注/Memo:
收稿日期:2005-1-2。
基金项目:国家自然科学基金(3983035)
作者简介:赵军(1974- ),女,解放军总医院在读硕士研究生,研究方向:子宫内膜异位症,电话:010-63822024
通讯作者:李亚里,北京解放军总医院妇产科,电话:010-66939292
更新日期/Last Update: 1900-01-01