[1]岳枫,马文丽,宋艳斌,等.RNAi技术沉默K562细胞中c-myc基因的初步研究[J].南方医科大学学报,2005,(06):647-650.
　YUE Feng,MA Wen-li,SONG Yan-bin,et al.c-myc gene silencing in K562 cells with RNA interference[J].Journal of Southern Medical University,2005,(06):647-650.
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RNAi技术沉默K562细胞中c-myc基因的初步研究()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年06期
页码:
647-650
栏目:
出版日期:
2005-06-01

文章信息/Info

Title:
c-myc gene silencing in K562 cells with RNA interference
作者:
岳枫1;  马文丽1;  宋艳斌1;  石嵘1;  张宝1;  郑文岭2
1. 南方医科大学分子生物学研究所, 广东, 广州, 510515;
2. 广州军区广州总医院医学实验科, 广东, 广州, 510010
Author(s):
YUE Feng1;  MA Wen-li1;  SONG Yan-bin1;  SHI Rong1;  ZHANG Bao1;  ZHENG Wen-ling2
1. Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China; 2. Department of Medical Experiment, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China
关键词:
RNAi; siRNA; 基因; myc; 逆转录聚合酶链反应; K562细胞
Keywords:
RNAi; siRNAs; gene; c-myc; reverse transcriptase polymerase chain reaction; K562 cells
分类号:
R394.2;R342.4
摘要:
目的 运用RNAi技术,设计针对c-myc的小干扰RNA(siRNA),研究其干扰效果。方法 针对c-mycmRNA的第1357靶位点设计siRNAs,经LipofectamineTM2000脂质体法转染K562细胞,进行RT-PCR、细胞计数和MTT法及流式细胞仪检测,观察其干扰效果。结果 转染组与阴性对照组和空白对照组相比,c-mycmRNA表达明显减弱,细胞计数和MTTD(λ)值均有显著降低,并有明显的凋亡率。结论 运用RNAi技术,可以有效地干扰c-myc的表达,并进一步诱导细胞凋亡。
Abstract:
Objective To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells. Methods siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamineTM 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting. Results Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate. Conclusion RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-10-26。
基金项目:国家自然科学基金(39880032)
作者简介:岳枫(1975- ),女,在读硕士研究生,电话:020-61640114-89097,E-mail:yuefeng@fimmu.com
通讯作者:马文丽,电话:020-61648210,E-mail:Wenli@fimmu.com
更新日期/Last Update: 1900-01-01