[1]江爱达,余林中,龚小卫,等.凉膈散药物血清对脂多糖诱导体外培养巨噬细胞核转录因子-кB的影响[J].南方医科大学学报,2005,(06):619-622.
 JIANG Ai-da,YU Lin-zhong,GONG Xiao-wei,et al.Effect of Liangge San on lipopolysaccharide-induced nuclear factor kappa B activation in cultured mouse macrophages[J].Journal of Southern Medical University,2005,(06):619-622.
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凉膈散药物血清对脂多糖诱导体外培养巨噬细胞核转录因子-кB的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年06期
页码:
619-622
栏目:
出版日期:
2005-06-01

文章信息/Info

Title:
Effect of Liangge San on lipopolysaccharide-induced nuclear factor kappa B activation in cultured mouse macrophages
作者:
江爱达1 余林中1 龚小卫2 邓鹏2 马晓冬3
1. 南方医科大学中医系, 广东, 广州, 510515;
2. 南方医科大学休克微循环实验室, 广东, 广州, 510515;
3. 南方医科大学中心实验室, 广东, 广州, 510515
Author(s):
JIANG Ai-da1 YU Lin-zhong1 GONG Xiao-wei2 DENG Peng2 MA Xiao-dong3
1. Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; 2. Key Laboratory of Shock and Microcirculation, Southern Medical University, Guangzhou 510515, China; 3. Central Laboratory, Southern Medical University, Guangzhou 510515, China
关键词:
凉膈散脂多糖巨噬细胞核转录因子-кB免疫荧光法激光扫描共聚焦显微镜
Keywords:
Liangge Sanlipopolysaccharidemacrophage nuclear factor-кBimmunofluorescencelaser scanning confocal microscope
分类号:
R289.5;R329.24;R394.2
摘要:
目的 观察凉膈散药物血清对脂多糖(LPS)诱导的体外培养的小鼠腹腔巨噬细胞核转录因子-кB(NF-кB)变化的影响,探讨凉膈散解毒作用的细胞信号转导调控机制。方法 制备凉膈散药物血清;提取并体外培养小鼠腹腔巨噬细胞;以凉膈散药物血清及LPS共同刺激已培养细胞,免疫荧光法检测巨噬细胞NF-кB亚基p65,用激光扫描共聚焦显微镜观察并测定各组小鼠腹腔巨噬细胞核的p65的荧光表达情况。结果 小鼠巨噬细胞经LPS刺激1h后,其核内的荧光强度(代表p65的表达量)显著增强。与LPS刺激组相比:抑制剂TLCK组、凉膈散药物血清不同剂量组的荧光强度值均较低,有显著性差异,以TLCK及凉膈散大剂量组强度值最低,中、小剂量组次之;空白血清不同剂量组则均无显著差异。药物血清不同剂量组之间有显著差异,呈剂量依赖性关系;空白血清不同剂量组之间无显著性差异。结论 不同剂量凉膈散药物血清均能抑制LPS所致的细胞核内p65升高,且呈剂量依赖性,这可能是凉膈散解毒作用的细胞信号转导机制之一。
Abstract:
Objective To investigate the effect of Liangge San, a recipe of traditional Chinese herbal medicine, on lipopolysac-charide (LPS)-induced nuclear factor кB (NF-кB) activation in mouse macrophages cultured in vitro and explore the signal transduction mechanism of the detoxifying effect of Liangge San. Methods The mice were given oral administration of concentrated decoction of Liangge San to obtain the drug-containing serum. Macrophages from mouse abdominal cavity were collected, incubated and subsequently re-incubated with LPS and the prepared serum at different doses. Immunofluorescence method was adopted to examine the expression of NF-кB subunit p65 in the nuclei of the macrophages, and the fluorescence intensity of p65 expression was measured by laser scanning confocal microscope (LSCM). Results The fluorescence intensity of p65 expression in the nuclei of macrophages incubated with LPS for 1 h was significantly increased compared with that in the cells without LPS stimulation and Liangge San serum-treated cells. The fluorescence intensities were significantly decreased in cells treated with the inhibitor TLCK and different doses of Liangge San serum in comparison with those in LPS-stimulated cells. The fluorescence intensities were the lowest in cells treated with TLCK and high-dose Liangge San serum, and the cells treated with moderate and low doses of the serum both showed lower intensity compared with that of LPS-stimulated cells. p65 expression was similar between the macrophages incubated with LPS and those treated with serum that contained no Liangge San. Conclusion Mouse serum containing Liangge San can inhibit LPS-induced p65 expression in mouse macrophages in a dose-dependent manner, which may be one of the signal transduction mechanisms of the detoxifying effect of Liangge San.

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备注/Memo

备注/Memo:
收稿日期:2005-1-31。
基金项目:国家自然科学基金(30171155)
作者简介:江爱达(1970- ),男,中医系在读硕士研究生
通讯作者:余林中,电话:020-61648262, E-mail:yulzh@fimmu.com
更新日期/Last Update: 1900-01-01