[1]邓星,王爽,黄吴键,等.氨基酸浓度对昆明小鼠胚胎体外发育的影响[J].南方医科大学学报,2005,(03):241-245.
 DENG Xing,WANG Shuang,HUANG Wu-jian,et al.Effects of different concentrations of amino acids in the culture medium on preimplantation mouse embryo development in vitro[J].Journal of Southern Medical University,2005,(03):241-245.
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氨基酸浓度对昆明小鼠胚胎体外发育的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年03期
页码:
241-245
栏目:
出版日期:
2005-03-01

文章信息/Info

Title:
Effects of different concentrations of amino acids in the culture medium on preimplantation mouse embryo development in vitro
作者:
邓星1 王爽1 黄吴键2 刘求真1 谢卫兵1 张庆凌1 方唯意3 刘腾飞3 韩春1 杜莎莎3 吴丽莎1 丁彦青1
1. 南方医科大学病理教研室肿瘤研究所, 广东, 广州, 510515;
2. 南方医科大学组织学与胚胎学教研室, 广东, 广州, 510515;
3. 中南大学肿瘤研究所, 湖南, 长沙, 410078
Author(s):
DENG Xing1 WANG Shuang1 HUANG Wu-jian2 LIU Qiu-zhen1 XIE Wei-bing1 ZHANG Qing-ling1 FANG Wei-yi3 LIU Teng-fei3 HAN Chun1 DU Sha-sha3 WU Li-sha1 DING Yan-qing1
1. Department of Pathology, Southern Medical University, Guangzhou 510515, China;
2. Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China;
3 Cancer Research Institute, Central South University, Changsha 410078, China
关键词:
小鼠植入前胚胎发育氨基酸
Keywords:
mouse Kunmingpreimplantation embryodevelopmentamino acid
分类号:
Q95-3
摘要:
目的 探讨培养液中氨基酸成分对植入前昆明小白鼠胚胎发育的影响,摸索植入前胚胎体外培养氨基酸的最佳浓度。方法 从超排昆明小鼠输卵管中取出的630枚受精卵均分为7组,分别在经添加不同浓度Eagle’s氨基酸的去牛血清白蛋白KSOM培养液(mKSOM、mKSOM+1/16AA、mKSOM+1/8AA、mKSOM+1/4AA、mKSOM+1/2AA、mKSOM+AA、mKSOM+2AA)中培养,对比观察各组胚胎体外发育的过程。用卡方检验分析添加各浓度氨基酸的培养液培养效率的差别,并将8细胞发育率和囊胚发育率分别与相应的氨基酸浓度进行曲线拟合分析。结果 与未添加氨基酸组对比,添加氨基酸组胚胎发育率高,8细胞胚和囊胚的形成率与培养液中氨基酸的浓度关系均符合三次模型,在氨基酸为1/2浓度时8细胞胚和囊胚的形成率均达到最高。结论 氨基酸对胚胎的体外发育有促进作用,但含有高浓度氨基酸的培养液培养效果反而有所下降,可能是由于高浓度的氨基酸影响胚胎的代谢和渗透压的改变,且氨基酸分解及胚胎代谢所产生的铵对胚胎有毒性作用。
Abstract:
Objective To evaluate the effects of amino acids (AA) on the development of in vitro cultured preimplantation embryos of Kunming mice, and define the optimal AA concentration for embryo culture.Methods Totally 630 zygotes were collected from the oviducts of superovulated female Kunming mice, which were cultured in protein-free potassium simplex optimized medium (mKSOM) supplemented with Eagle’s essential amino acids and Eagle’s non-essential amino acids of different concentrations (mKSOM, mKSOM+1/16AA, mKSOM+1/8AA, mKSOM+1/4AA, mKSOM+1/2AA, mKSOM+AA, and mKSOM+2AA).Results The embryos cultured with the amino acids showed higher development rate to both 8-cell embryo stage and blastocyst stage than those cultured without amino acids. The correlation of amino acid concentration with 8-cell and blastocyst development rates conformed to the cubic model, with the highest development rate to both of the two stages observed at half of the amino acid concentration.Conclusion Amino acids can promote the development of preimplantation Kunming mouse embryos, but excessively high concentration of amino acids impair embryo development possibly because of metabolic and osmotic pressure changes of the embryos as well as toxicity of ammonium resulting from the metabolism of amino acids.

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备注/Memo

备注/Memo:
收稿日期:2004-5-6。
基金项目:National Natural Science Foundation of China (39730200)
更新日期/Last Update: 1900-01-01