[1]刘欣,刘国炳,庞战军,等.PCR直接测序法检测宫颈癌组织中人乳头瘤病毒DNA[J].南方医科大学学报,2005,(02):201-203.
 LIU Xin,LIU Guo-bing,PANG Zhan-jun,et al.Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing[J].Journal of Southern Medical University,2005,(02):201-203.
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PCR直接测序法检测宫颈癌组织中人乳头瘤病毒DNA()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2005年02期
页码:
201-203
栏目:
出版日期:
2005-02-01

文章信息/Info

Title:
Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing
作者:
刘欣1 刘国炳1 庞战军1 邢福祺1 郭遂群1 马文丽2 郑文岭2
1. 南方医科大学南方医院妇产科, 广东, 广州, 510515;
2. 南方医科大学分子生物学研究所, 广东, 广州, 510515
Author(s):
LIU Xin1 LIU Guo-bing1 PANG Zhan-jun1 XING Fu-qi1 GUO Sui-qun1 MA Wen-li2 ZHENG Wen-ling2
1. Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;
2. Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China
关键词:
人类乳头瘤病毒聚合酶链反应测序分析宫颈癌
Keywords:
human papillomaviruspolymerase chain reactionsequence analysiscervical cancer
分类号:
R373.9
摘要:
目的 探索检测宫颈癌组织中人乳头瘤病毒(HPV)DNA的有效手段。方法 采用HPV通用引物对宫颈癌标本中的HPVL1区基因序列进行PCR扩增,根据PCR产物序列分析对HPV分型。结果 50例宫颈癌组织HPVDNA检出率为78%,其中HPV16和HPV18型混合感染最多见。检测出1例HPV58型,其L1区基因序列与德国标准株58型比对,未发现碱基变异。结论 PCR直接测序法是对宫颈癌组织中HPV检测和分型的一种有效方法,并可检测到HPV少见的特殊类型,同时也能提供已知的HPV型别的突变信息。
Abstract:
Objective To explore an effective method for detecting human papillomavirus(HPV) DNA in cervical cancer tissue. Methods HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product. Results The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections. Infection with HPV58 was detected in one case. The sequencing results showed no difference in L1 sequence between the detected samples and the standard German HPV58 strain. Conclusion PCR and direct sequencing approach is effective for detecting and typing of HPV DNA in cervical cancer tissue, through which rare HPV strain or mutants of known HPV strains may not escape detection.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-8-11。
基金项目:南方医院2002年新技术新业务课题
作者简介:刘欣(1975- ),女,1998年毕业于同济医科大学,在读硕士研究生,电话:020-61365788,E-mail:liuxin23@fimmu.com
更新日期/Last Update: 1900-01-01