[1]李志清,黄跃生,杨宗城,等.烧伤血清对内皮细胞IκBα降解和NF-κB活化的影响[J].南方医科大学学报,2005,(02):181-183.
 LI Zhi-qing,HUANG Yue-sheng,YANG Zong-cheng,et al.Effects of burn patients’ serum on IκBα degradation and NF-κB activation in endothelial cells in vitro[J].,2005,(02):181-183.
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烧伤血清对内皮细胞IκBα降解和NF-κB活化的影响()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年02期
页码:
181-183
栏目:
出版日期:
2005-02-01

文章信息/Info

Title:
Effects of burn patients’ serum on IκBα degradation and NF-κB activation in endothelial cells in vitro
作者:
李志清1 黄跃生2 杨宗城2 王甲汉1
1. 南方医科大学南方医院烧伤科, 广东, 广州, 510515;
2. 第三军医大学西南医院烧伤研究所, 重庆, 400038
Author(s):
LI Zhi-qing1 HUANG Yue-sheng2 YANG Zong-cheng2 WANG Jia-han1
1. Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Chine;
2. Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
烧伤/血液内皮细胞NF-κB抑制性κB
Keywords:
burns/bloodendothelial cellnuclear factor-κBinhibiting κBα
分类号:
R644
摘要:
目的 了解烧伤血清对内皮细胞抑制性κB(IκBα)降解、核因子-κB(NF-κB)活化的影响,进一步探讨烧伤血清诱导内皮细胞活化分泌细胞因子的机制。方法 采用体外培养的内皮细胞,分别用正常人血清(对照组)、烧伤患者血清、烧伤患者血清+吡咯烷二硫代氨基甲酸盐(PDTC)刺激内皮细胞。采用Western印迹法检测血清刺激30、60、90、120min后内皮细胞IκBα蛋白降解情况,电泳迁移率分析检测血清刺激30、60、120、240min后NF-κB活性的变化。结果 烧伤血清刺激内皮细胞后30min,IκBα发生明显降解,刺激后60min达高峰,2h后表达逐渐升高;烧伤血清刺激内皮细胞后30min,NF-κB活性迅速升高,30~60min达高峰,2h后逐渐回复基础状态。PDTC能有效抑制烧伤血清作用条件下内皮细胞IκBα降解、NF-κB活化。结论 烧伤血清可诱导内皮细胞IκBα降解,活化NF-κB,从而在烧伤后内皮细胞分泌细胞因子过程中起重要作用。
Abstract:
Objective To investigate the effects of the serum from burned patients(burn serum) on inhibitor of κBα(IκBα) degradation and nuclear factor-κB(NF-κB) activation in the endothelial cells in order to explore the role of burn serum on endothelium activation. Methods Cultured endothelial cells(ECV-304) were stimulated by the serum from healthy volunteers, burn serum and burn serum with pyrrolidine dithiocarbamate(PDTC), respectively. Activation of endothelial NF-κB at 30, 60, 120, 240 min after stimulation was tested by electrophoretic mobility shift assay(EMSA) and the degradation of endothelial IκBα at these time points determined by Western blotting. Results Compared with that in the control group, cytosolic IκBα degradation in the endothelial cells occurred within 30 min after burn serum stimulation, and peaked at 60 min, followed by gradual recovery in the cytoplasm till reaching the pre-stimulation level at 2 h. Meanwhile, the activity of endothelial NF-κB was markedly increased, reaching the peak at 30 to 60 min with subsequent gradual decrease 2 h after stimulation. PDTC could effectively inhibit endothelial IκBα degradation and activation of NF-κB induced by the burn serum. Conclusion Burn serum might induce the degradation of IκBα to activate NF-κB, which ultimately leads to cytokine release from the endothelium.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-9-23。
基金项目:国家重点基础研究发展规划基金(G1999054202)
作者简介:李志清(1967- ),男,博士,主治医师,电话:020-61647627
更新日期/Last Update: 1900-01-01