[1]柳太云,张成,肖露露,等.人脐血单个核细胞诱导分化为神经元样细胞[J].南方医科大学学报,2005,(02):152-155.
 LIU Tai-yun,ZHANG Cheng,XIAO Lu-lu,et al.Induced differentiation of human cord blood monocytes into neuron-like cells in vitro[J].,2005,(02):152-155.
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人脐血单个核细胞诱导分化为神经元样细胞
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年02期
页码:
152-155
栏目:
出版日期:
2005-02-01

文章信息/Info

Title:
Induced differentiation of human cord blood monocytes into neuron-like cells in vitro
作者:
柳太云1 张成1 肖露露2 姚晓黎1 冯善伟1 曾缨1
1. 中山大学附属第一医院神经内科, 广东, 广州, 510089;
2. 广州血液中心干细胞室, 广东, 广州, 510095
Author(s):
LIU Tai-yun1 ZHANG Cheng1 XIAO Lu-lu2 YAO Xiao-li1 FENG Shan-wei1 ZENG Ying1
1. Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510089, China;
2. Laboratory of Stem Cells, Guangzhou Blood Center, Guangzhou 510095, China
关键词:
脐血间充质干细胞神经元样细胞诱导分化
Keywords:
cord bloodmesenchymal stem cellsneuro-like cellsinduced differentiation
分类号:
R329.24
摘要:
目的 探讨人脐血单个核细胞体外向神经元样细胞定向诱导分化的条件。方法 无菌条件下采集正常人脐血,分离单个核细胞。用DMEM/F12培养基进行纯化和扩增培养。取扩增5代的间充质干细胞(MSC),分别用β-巯基乙醇(β-ME)、二甲基亚砜(DMSO)、神经条件培养液诱导脐血MSC向神经元样细胞分化。免疫组化法检测脐血MSC的Nestin表达和神经元样细胞特异性标志。结果 脐血原代和2、5代MSC的Nestin表达阳性率分别为6.7%、12.4%和20.8%。神经条件培养液诱导神经元样细胞阳性率最高达36%,β-ME、DMSO分别为33%和25%。结论 脐血MSC具有神经干/祖细胞的特性,在一定条件下能向神经元样细胞分化。
Abstract:
Objective To probe the conditions for inducing human cord blood monocytes to differentiate into neuron-like cells. Methods The mononuclear cells were isolated from human umbilical cord blood samples and plated in 25-mm culture flasks containing DMEM/F12 medium. The fifth passage of mesenchymal stem cells(MSCs) were induced by β-mercaptoe-thanol(β-ME), dimethyl sulfoxide(DMSO) and conditioned medium for neuron induction, respectively, to differentiate into neuron-like cells. The expression of nestin, neuron-specific enolase(NSE) and neurofilament(NF) on the treated cells were detected by immunocytochemical method. Results Nestin expression were found in 6.7% of the primary, 12.4% of the second passage, 20.8% of the fifth passage of MSCs, respectively. Thirty-six percent of cell cultures treated with the conditioned medium for neuron induction were immunoreactive for NSE and NF, and 33% of the cells induced by β-ME and 25% of the cells by DMSO also expressed NSE and NF. Conclusion Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-10-15。
基金项目:卫生部临床学科重点项目(2001321);国家自然科学基金(30170337)
作者简介:柳太云(1964- ),男,副主任医师,在读博士研究生,从事神经遗传性疾病研究,电话:020-87334192,E-mail:ltylxlf@gzsums.edu.cn
通讯作者:张成,电话:020-86332387;E-mail:czym@gzsums.edu.cn
更新日期/Last Update: 1900-01-01