[1]汪群力,裴国献,曾宪利,等.恒河猴骨髓基质干细胞与新型可吸收羟基磷灰石及β-磷酸三钙体外相容性的观察[J].南方医科大学学报,2005,(01):44-47.
 WANG Qun-li,PEI Guo-xian,ZENG Xian-li,et al.In vitro biocompatibility of novel absorbable hydroxyapatite and AO artificial bone β-tricalcium phosphate with rhesus bone marrow stromal cells[J].,2005,(01):44-47.
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恒河猴骨髓基质干细胞与新型可吸收羟基磷灰石及β-磷酸三钙体外相容性的观察()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年01期
页码:
44-47
栏目:
出版日期:
2005-01-01

文章信息/Info

Title:
In vitro biocompatibility of novel absorbable hydroxyapatite and AO artificial bone β-tricalcium phosphate with rhesus bone marrow stromal cells
作者:
汪群力1 裴国献1 曾宪利1 金丹1 魏宽海1 刘晓霞1 钟世镇2 欧阳钧2
1. 南方医科大学南方医院创伤骨科, 广东广州510515;
2. 广东省组织构建与检测重点实验室, 广东广州510515
Author(s):
WANG Qun-li1 PEI Guo-xian1 ZENG Xian-li1 JIN Dan1 WEI Kuan-hai1 LIU Xiao-xia1 ZHONG Shi-zhen2 OU Yang-jun2
1. Department of Traumatology and Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;
2. Guangdong Key Laboratory of Tissue Construction and Examination, Guangzhou 510515, China
关键词:
组织工程骨髓基质干细胞生物材料生物相容性
Keywords:
tissue engineeringbone marrow stromal cellbiomaterialbiocompatibility
分类号:
R68
摘要:
目的 观察恒河猴骨髓基质干细胞(rBMSC)与新型可吸收羟基磷灰石(HA)及AO人工骨茁-磷酸三钙(茁-TCP)的体外相容性。为在灵长类动物体内构建组织工程化骨作材料方面的准备。方法 取第三代恒河猴骨髓基质细胞与材料复合培养。实验分3组:A组将rBMSC与HA复合培养;B组rBMSC与茁-TCP复合培养;C组为对照,只有细胞不加材料。倒置相差显微镜、扫描电镜观察各组细胞形态及增殖情况,MTT法半定量检测细胞增殖。结果 倒置显微镜下见HA组细胞生长良好,与对照组无显著差异。茁-TCP组有一些细小颗粒脱落散在分布于板底或细胞表面,有少量细胞脱落死亡。扫描电镜见HA组细胞贴附、增殖良好,茁-TCP组细胞贴附率不高。MTT法显示HA组细胞增殖与对照组接近,而茁-TCP组则显著低于对照组。结论 新型HA与恒河猴BMSc生物相容性好,可用作恒河猴骨组织工程的支架材料,AO人工骨需要作性能上的改进。
Abstract:
Objective To study the in vitro biocompatibility of novel hydroxyapatite (HA) and AO artificial bone β-tricalcium phosphate (β-TCP) with rhesus bone marrow stromal cells (rBMSCs). Methods The third passage of rBMSCs were cultured with HA and β-TCP respectively, with the cells cultured without the materials as the control. The morphology and proliferation of cells were observed by inverted phase-contrast microscope and scanning electron microscope (SEM). MTT assay was used to semiquantitatively evaluate the cell proliferation. Results The rBMSC cocultured with HA exhibited good growth as observed under inverted phase-contrast microscope, without significant difference from the cells in the control group. Some small particles were seen pealing off from β-TCP, and some of the cells died. Under SEM, rBMSCs showed good adhesion to HA with obvious proliferation, but the ratio of adhesive cells was not as high as that in β-TCP group. MTT assay showed no significant difference in the cell number between HA and the control groups, but the cell number in β-TCP group was notably less than that of control group. Conclusion Novel HA has good biocompatibility with rBMSCs for bone tissue engineering, and AO artificial bone still needs improvement to serve as scaffold material for BMSCs.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-6-4。
基金项目:国家高技术发展计划(863计划)重大专项(2003AA205010);国家重点基础研究发展规划(973项目)子课题(G19990543-09);广州市科委科技攻关重点项目(200221-0038);国家自然科学基金(30300367)
作者简介:汪群力(1973- )男,1996年毕业于第三军医大学,博士研究生,研究方向:骨组织工程,电话:020-61641744,E-mail:wq1235@fimmu.com
更新日期/Last Update: 1900-01-01