[1]武大林,凌汉新,唐浩.PCR-SSP法HLA-A、B基因分型与血清学分型的比较[J].南方医科大学学报,2004,(11):1267-1270.
 WU Da-lin,LING Han-xin,TANG Hao.Comparative studies of serological typing and HLA-A, B antigen genotyping with PCR using sequence-specific primers[J].Journal of Southern Medical University,2004,(11):1267-1270.
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PCR-SSP法HLA-A、B基因分型与血清学分型的比较()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年11期
页码:
1267-1270
栏目:
出版日期:
2004-11-01

文章信息/Info

Title:
Comparative studies of serological typing and HLA-A, B antigen genotyping with PCR using sequence-specific primers
作者:
武大林1 凌汉新2 唐浩1
1. 南方医科大学南方医院医学中心实验科, 广东, 广州, 510515;
2. 广州市第十二人民医院肾内科, 广东, 广州, 510620
Author(s):
WU Da-lin1 LING Han-xin2 TANG Hao1
1. Central Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;
2. Department of Nephrology, Twelfth People’s Hospital of Guangzhou, Guangzhou 510620, China
关键词:
HLA抗原聚合酶链反应序列特异性引物单克隆抗体等位基因分型
Keywords:
HLA antigenspolymeras chain reactionsequence-specific primersmonoclonal antibodyallele typing
分类号:
R446.11
摘要:
目的 比较聚合酶链反应-序列特异性引物(PCR-SSP)进行HLA-Ⅰ类A、B抗原位点分型的准确性,并探讨血清学分型错误发生的原因。方法 用PCR-SSP以及单克隆抗体血清学分型技术对HLA-A、B分型并比较。结果 34例样本PCR-SSP基因分型无假阳性和假阴性出现。PCR-SSP法与血清学比较,血清学检出错误或漏检率分别为HLA-A位点23.5%,B位点26.5%。血清学发生错误或易混淆的抗原有:A2和A68、A32和A33,B5、B60和61。结论 PCR-SSP法进行HLA-A、B抗原等位基因分型具有分辨率高、特异性强、重复性好、实验过程简捷快速、分型结果较血清学更加准确可靠的优点。
Abstract:
Objective To evaluate the accuracy of PCR with sequence-specific primers (PCR-SSP) for HLA-Ⅰ genotyping and analyze the causes of the errors occurring in the genotyping.Methods DNA samples and were obtained from 34 clinical patients, and serological typing with monoclonal antibody (mAb) and HLA-A and, B antigen genotyping with PCR-SSP were performed.Results HLA-A and, B alleles were successfully typed in 34 clinical samples by mAb and PCR-SSP. No false positive or false negative results were found, and the erroneous and missed diagnosis rates were obviously higher in serological detection, being 23.5% for HLA-A and 26.5% for HLA-B. Error or confusion was more likely to occur in the antigens of A2 and A68, A32 and A33, B5, B60 and B61.Conclusion s DNA typing for HLA-Ⅰclass (A, B antigens) by PCR-SSP has high resolution, high specificity, and good reproducibility, which is more suitable for clinical application than serological typing. PCR-SSP may accurately detect the alleles that are easily missed or mistaken in serological typing.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2003-5-26。
基金项目:第一军医大学南方医院1998新技术攻关项目(98字第011号)
作者简介:武大林(1954- ),女,1986年毕业于第三军医大学,主任检验技师,电话:020-85141042,E-mail:wksys@fimmu.com>
更新日期/Last Update: 1900-01-01