[1]沈青,姚裕家,李如英,等.斑蝥素对G1/S期肾小管上皮细胞ATP的调节[J].南方医科大学学报,2004,(10):1192-1193,1196.
 SHEN Qing,YAO Yu-jia,LI Ru-ying,et al.Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells[J].,2004,(10):1192-1193,1196.
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斑蝥素对G1/S期肾小管上皮细胞ATP的调节()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2004年10期
页码:
1192-1193,1196
栏目:
出版日期:
2004-10-01

文章信息/Info

Title:
Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells
作者:
沈青1 姚裕家2 李如英1 丁翠玲1 侯立朝3 孙绪德3
1. 第四军医大学西京医院儿科, 陕西, 西安, 710032;
2. 四川大学华西二院儿科, 四川, 成都, 610041;
3. 第四军医大学西京医院麻醉科, 陕西, 西安, 710032
Author(s):
SHEN Qing1 YAO Yu-jia2 LI Ru-ying1 DING Cui-ling1 HOU Li-chao3 SUN Xu-de3
关键词:
肾小管上皮细胞斑蝥素三磷酸腺苷肌动蛋白类低氧
Keywords:
renal tubule epithelial cellscantharidinadenosine triphosphateactinshypoxia
分类号:
R364.1;R329.2
摘要:
目的 通过斑蝥素对G1/S期肾小管上皮细胞(RTECs)低氧损伤后细胞ATP代谢影响的研究,探讨斑蝥素稳定肌动蛋白微丝骨架系统的机制。方法 将新生猪G1/S界面RTECs分为3组:实验组(氰化钠+斑蝥素)、缺氧组(氰化钠)和空白对照组,应用反相高效液相比较缺氧后各组细胞内ATP含量的变化。结果 实验组细胞内ATP含量[(14.50±0.26)mmol/g蛋白]明显高于缺氧组[(4.25±0.11)mmol/g蛋白]和空白对照组[(8.58±0.13)mmol/g蛋白](P<0.01)。结论 斑蝥素稳定肌动蛋白微丝骨架系统可能与抗RTECs低氧损伤后细胞能量代谢紊乱相关。
Abstract:
Objectives To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G1/S-phase renal tubule epithelial cells (RTECs).Methods G1-phase RTECs were divided into cantharidin-treated group,exposed to sodium cyanide (CN) and cantharidin,hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography. Results The concentration of CN exposure for 1 h,ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50±0.26 mmol/g protein,4.25±0.11 mmol/g protein,8.58±0.13 mmol/g protein,respectively,P<0.01).Conclusion Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2004-2-18。
作者简介:沈青(1963- ),男,医学博士,副主任医师,电话:029-3375399,E-mail:mailsq@fmmu.edu.cn
更新日期/Last Update: 1900-01-01