[1]李斌.体外三维抗血管生成药物筛选模型的建立[J].南方医科大学学报,2004,(10):1167-1170.
 LI Bin.Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection[J].Journal of Southern Medical University,2004,(10):1167-1170.
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体外三维抗血管生成药物筛选模型的建立()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年10期
页码:
1167-1170
栏目:
出版日期:
2004-10-01

文章信息/Info

Title:
Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection
作者:
李斌
云南大学药学院, 云南昆明650091
Author(s):
LI Bin
关键词:
内皮细胞血管生成模型药物筛选
Keywords:
angiogenesis modelendothelial cellsdrug selection
分类号:
R733.7
摘要:
目的 建立一种新的用于筛选具有抗血管生成作用的体外血管生成模型。方法 人脐静脉的内皮细胞(HUVECs)在三维胶原纤维系统中培养,制备成具有毛细血管样网状结构模型;3种酪氨酸酶抑制剂genistein,tyrphostinA23和lavendustinC被分别加入到模型中,观察它们对血管形成的影响。结果 在不加入任何生长因子的条件下,HUVECs能迅速重组形成毛细血管样网状结构;3种酪氨酸酶抑制剂均对血管形成有抑制作用。结论 该模型具有操作简单、可重复性好、易量化的特点,是一种在体外筛选血管生成抑制剂的理想模型。
Abstract:
Objective To develop a simple human angiogenesis model that simulates all the critical steps of the angiogenic process for screening antiangiogenic substances.Methods Human umbilical vein endothelial cells (HUVECs) were sandwiched into three-dimensional collagen gels for culturing to produce the angiogenesis model in vitro,on which the antiangiogenic effects of genistein,tyrphostin A23 and lavendustin C were assessed. Results HUVECs had the capacity to repidly form highly organized capillary-like structures in this model with uniformity of the entire culture,and was easily reproducible. Tyrosine kinase inhibitors,genistein,tyrphostin A23 and lavendustin C were found to inhibit the capillary tube formation.Conclusion This new human angiogenesis model has proved to be simple,responsive,and reproducible for testing potential angiogenic inhibitors,and is suitable for screening anti-angiogenic drugs.

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备注/Memo

备注/Memo:
收稿日期:2004-2-16。
基金项目:云南大学理(工)科校级科研项目资助(K1059069)
作者简介:李斌(1967- ),女,2002年毕业于英国UniversityofBradford,硕士,助研,电话:0871-5031119,E-mail:libin36@ynu.edu.cn
更新日期/Last Update: 1900-01-01