[1]李志峰,聂军,戴迎春,等.副溶血弧菌不耐热溶血毒素tlh基因的序列分析[J].南方医科大学学报,2004,(10):1153-1155.
 LI Zhi-Feng,NIE Jun,DAI Ying-Chun,et al.Bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene[J].Journal of Southern Medical University,2004,(10):1153-1155.
点击复制

副溶血弧菌不耐热溶血毒素tlh基因的序列分析()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年10期
页码:
1153-1155
栏目:
出版日期:
2004-10-01

文章信息/Info

Title:
Bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene
作者:
李志峰 聂军 戴迎春 李建栋 陈清 俞守义
南方医科大学流行病学教研室, 广东, 广州, 510515
Author(s):
LI Zhi-Feng NIE Jun DAI Ying-Chun LI Jian-dong CHEN Qing YU Shou-yi
关键词:
弧菌副溶血性碱基序列聚合酶链反应溶血素类
Keywords:
Vibrio parahaemolyticusbase sequencepolymerase chain reactionhemolysins
分类号:
R378.3;R394.2
摘要:
目的 利用PCR技术扩增副溶血弧菌不耐热溶血毒素tlh基因,对tlh基因进行生物信息学分析。方法 利用PCR技术扩增tlh基因,克隆至载体pET32a+ 并测序。将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。结果和结论 测序结果tlh14-90被GenBank收录,登录号为AY289609。tlh14-90全长1257bp,与国际标准株WP1的同源性为99%,含有启动子和密码子,预测其编码一个含418个氨基酸的蛋白质(TLH14-90),分子式为C2131H3184N548O649S16,相对分子质量47392.9,理论等电点pI为4.92,丙氨酸、亮氨酸和天冬酰氨的含量分别为11.0%、7.4%和7.2%,含有4个半胱氨酸(Cys),κ-D法推算的疏水性参数为-34。预测其二级折叠结构为α/□蛋白,三级结构未给出。
Abstract:
Objective To carry out bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene(tlh) obtained by PCR amplification.Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced,followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.Results and Conclusions The sequenced tlh gene (named tlh14-90) was entered into GenBank with the accession number of AY289609. Tlh14-90 has a length of 1 257 bp with both start and stop codons,having 99% homology with the tlh gene of WP1. Tlh14-90 is predicted to encode a protein containing 418 amino acids (named TLH14-90,with the molecular formula of C2131H3184N548O649S16,molecular weight of 47 392.9,and the theoretical PI of 4.92). This protein consists of 4 Cys and the contents of Ala,Leu and Asn are 11.0%,7.4% and 7.2%,respectively,having a hydrophobic parameter of -34 calculated using κ-D method. Predicted as a α/□protein for its secondary structure,TLH14-90 has not been identified for its tertiary structure.

参考文献/References:

[1] Miyamoto Y,Kato T,Obara Y,et al.In vitro hemolytic characteristic of Vibrio parahaemolyticus:its close correlation with human pathogenicity[J].J Bacteriol,1969,100(2):1147-9.
[2] Okuda JM,Ishibashi E,Hayakawa T,et al.Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta,India,and isolation of strains from the same clonal group from Southeast Asia travelers arriving in Japan[J].J Clin Microbiol,1997,35(12):3150-5.
[3] Takahashi A,Kenjyo N,Imura K,et al.Cl(-) secretion in colonic epithelial cells induced by the Vibrio parahaemolyticus hemolytic toxin related to thermostable direct hemolysin [J].Infect Immun,2000,68(9):5435-8.
[4] Qiao HL,Zhang J.Test and assay the pathogenicity of Vibrio parahaemolyticus [J].Aquat Sci,1999,18(4):29-32.
[5] Taniguchi H,kubomura S,Hirano H,et al.Cloning and characterization of gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus [J].FEMS Microbiol Lett,1990,55 (3):339-45.
[6] Yanagase Y,Inoue K,Ozaki M,et al.Hemolysins and related enzymes of Vibrio parahaemolyticus.I.Identification and partial purification of enzymes[J].Biken J,1970,13(2):77-92.
[7] 李志峰,聂军.副溶血弧菌tlh基因克隆载体和表达载体的构建[J].中国公共卫生,2003,19(4):418-9.Li ZF,Nie J.Construction of clone and expression vector of gene of Vibrio parahaemolyticus [J].Chin J Pub Health,2003,19(4):418-9.
[8] 苏建新,聂军,吴振龙.副溶血性弧菌耐热直接相关溶血素基因的克隆与序列分析[J].第一军医大学学报,2002,22(6):515-7.Su JX,Nie J,Wu ZL.Cloning and sequence analysis of thermostable direct hemolysin-related hemolysin gene of Vibrio parahaemolyticus[J].J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao,2002,22(6):515-7.
[9] 欧阳曙光,贺福初.生物信息学:生物实验数据和计算技术结合的新领域[J].科学通报(Sci Bull),1999,44(14):1457-68.
[10] Shinoda S,Matsuoka H,Tsuchie T,et al.Purification and characterizaton of a lecithin-haemolysin from Escherichia coli transformed by a Vibrio parahaemolyticus gene[J].J Gen Microobiol,1991,137(Pt 12):2705-11.
[11] 李明,谢毅,李英杰,等.恶性疟原虫海南、云南、安徽株裂殖子表面抗原MSA2基因的克隆和序列分析[J].第一军医大学学报,1995,15(2):94-8.Li M,Xie Y,Li Y J,et al.Cloning and sequence analysis of the gene encoding the second region of MSA1 from three Chinese isolates[J].J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao,1995,15(2):94-8.
[12] Ellison RK,Malnati E,Depaloa A,et al.Population of vibrio parahoemolyticus in retail oysters from Florida using two metods[J].J Food Prot,2001,64(5):682-6.
[13] Myers ML,Panicker G,Bej AK.PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the gulf of Mexico [J].Appl Environ Microbiol,2003,69(4):2194-200.
[14] Iida T,Park KS,Suthienkul O,et al.Close proximity of tdh,trh and ure genes on the chromosone of Vibrio parahaemolyticus [J].Microbiology,1998,144(pt 9):2517-23.
[15] Takahasi A,Kenjyo N,Imura K,et al.Secretion in colonic epithelial cell induced by the vibrio parahaemalyticus hemolytic toxin related to thermostable direct hemolysin [J].Infec Immun,2000,68(9):5435-8.

备注/Memo

备注/Memo:
收稿日期:2004-3-8。
基金项目:全军十五指令性课题(01L050)
作者简介:李志峰(1970- ),男,在读博士研究生,助理研究员,电话:020-61648311,E-mail:sdlizf@163.com
更新日期/Last Update: 1900-01-01