[1]姜耀东,郑少斌,王战会.RT-PCR法检测肾癌组织中MN/CAⅨ基因表达[J].南方医科大学学报,2004,(08):925-927.
 JIANG Yao-dong,ZHENG Shao-bin,WANG Zhan-hui.MN/CAⅨ gene expression in renal cell carcinoma detected by RT-PCR[J].Journal of Southern Medical University,2004,(08):925-927.
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RT-PCR法检测肾癌组织中MN/CAⅨ基因表达()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年08期
页码:
925-927
栏目:
出版日期:
2004-08-01

文章信息/Info

Title:
MN/CAⅨ gene expression in renal cell carcinoma detected by RT-PCR
作者:
姜耀东1 郑少斌1 王战会2
1. 第一军医大学南方医院泌尿外科, 广东, 广州, 510515;
2. 第一军医大学南方医院感染内科实验室, 广东, 广州, 510515
Author(s):
JIANG Yao-dong1 ZHENG Shao-bin1 WANG Zhan-hui2
1. Department of Urology, First Military Medical University, Guangzhou 510515, China;
2. Department of Infection, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China
关键词:
聚合酶链反应肾细胞癌基因表达MN/CAⅨ基因
Keywords:
polymerase chain reactionrenal cell carcinomagene expressionMN/CAⅨgene
分类号:
R737.11
摘要:
目的 评价RT-PCR法检测MN/CAⅨ基因在肾癌组织表达的可靠性。方法 以5例肾癌和5例正常肾组织为研究对象,提取总RNA,两步法进行RT-PCR反应,用MN/CAⅨ的特异性引物扩增,2%琼脂糖凝胶电泳后观察并测序。结果 经过测序证实5例肾癌组织的RT-PCR产物中有4例可见特异的MN/CAⅨ条带,而正常肾组织的RT-PCR产物均无相应的MN/CAⅨ条带。结论 RT-PCR法检测MN/CAⅨ在肾癌组织中的表达是准确、可靠的。
Abstract:
Objective To evaluate the reliability of reverse transcriptional (RT)-PCR in detecting MN/CAⅨ gene expression in renal cell carcinoma (RCC). Methods MN/CAⅨ gene expression was detected in 5 RCC tissues and 5 normal renal tissues. The primers of MN/CAⅨ gene were designed for a two-step RT-PCR, and the products examined by 2% agarose gel electrophoresis and identified by sequence analysis. Result Corresponding bands of MN/CAⅨ gene could be seen in RT-PCR products of cases RCC tissue from 4 but not in normal renal tissues. Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2003-11-29。
作者简介:姜耀东(1974-),男,第一军医大学在读硕士研究生,电话:020-61647097
更新日期/Last Update: 1900-01-01