[1]朴英杰.去分化与细胞组织损伤再生[J].南方医科大学学报,2004,(07):736-737.
 PIAO Ying-jie.Dedifferentiation and regeneration of damaged cells and tissues[J].Journal of Southern Medical University,2004,(07):736-737.
点击复制

去分化与细胞组织损伤再生()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年07期
页码:
736-737
栏目:
出版日期:
2004-07-01

文章信息/Info

Title:
Dedifferentiation and regeneration of damaged cells and tissues
作者:
朴英杰
第一军医大学解剖学教研室, 广东, 广州, 510515
Author(s):
PIAO Ying-jie
Department of Anatomy, First Military Medical University, Guangzhou 510515, China
关键词:
去分化自噬胞质脱落胞质脱落体再生
Keywords:
dedifferentiationautophagyapocytosisapocytosomeregeneration
分类号:
R329.28
摘要:
去分化是发育生物学和形态学领域一个古老又常新的现象,既往研究多注重去分化和肿瘤发生的关系。我们的系列研究发现去分化和细胞组织损伤再生有着重要和必然的联系,在周围神经损伤再生中发现局部有大量施万细胞通过自噬机制吞噬消化自身髓鞘成分和衰老的细胞器,同时以胞质脱落的方式芽生出部分胞质脱落体,细胞内含大量游离核糖体和多聚核糖体等原始细胞器,保留极少量有活力的线粒体和高尔基体,呈现去分化后的幼稚化状态。在长春新碱诱导L02(肝细胞株)细胞损伤实验中发现L02细胞中有大量的自噬体,细胞周围也出现大量发芽脱落后游离于细胞外的胞质脱落体,细胞耐过之后呈现幼稚化,我们也称之为去分化。细胞经过去分化减轻代谢负担,清除衰老和受损的细胞器,恢复活力,接着大量增殖以修复重建损伤组织。在当前大部分科研人员一致看好干细胞在组织损伤再生中的重要作用的背景下,本研究根据长期观察实践,提出成熟细胞去分化幼稚化参与再生重建的观点,以供同行专家讨论。
Abstract:
Dedifferentiation is an important event in developmental biology and morphology but frequently neglected by the biologists. In the past, attention was given predominantly to its relationship with tumorigenesis. In our series of researches, we found important and inherent relations between dedifferentiation and regeneration of damaged cells and tissues in the emergence of large numbers of Schwarm cells that digested their own myelin sheath components and senescent organelles by autophagic mechanism during regeneration of the peripheral nerves following injuries, when some apocytosomes (apo+cyto+sis), by means of apocytosis (apo+cyto+sis), budded from the cells containing such primitive organelles as free ribosomes and polyribosomes and retaining a very small quantity of active mitochondria and Golgi apparatus, all characterizing an immature state of the cells after dedifferentiation. Many autophagic bodies were found enwrapped by membranes L02 cells (a liver cell line) after vincristine-induced damage, with numerous apocytosomes dissociated following budding around the cells. The cells survived the treatment presented immature appearance, a phenomenon we termed as dedifferentiation, after which the metabolic burden of the cells was relieved, the senescent and damaged organelles were cleared, and the cells resumed their viability and proliferated vigorously to repair and reconstruct the damaged tissues. As most researchers have currently give their full attention to the important role of stem cells in the regeneration of damaged cells and tissues, we, after long-term observations and experiments, propose that mature cells also take part in the regeneration and reconstruction process after dedifferentiation.

相似文献/References:

[1]李锦,白雪源,崔少远,等.雷帕霉素对高糖诱导的肾系膜细胞自噬抑制、氧化损伤和衰老的影响[J].南方医科大学学报,2012,(04):467.
[2]朴仲贤,王万山,徐锡金,等.大鼠坐骨神经再生过程中的神经元轴突自噬作用[J].南方医科大学学报,2004,(04):361.
 PIAO Zhong-xian,WANG Wan-shan,XU Xi-jin,et al.Autophagy of neuron axon during regeneration of rat sciatic nerves[J].Journal of Southern Medical University,2004,(07):361.
[3]王万山,朴仲贤,韩明虎,等.大鼠坐骨神经再生过程中的施万细胞自噬作用[J].南方医科大学学报,2004,(01):85.
 WANG Wan-shan,PIAO Zhong-xian,HAN Ming-hu,et al.Autophagic effect of Schwann cells in the regeneration of rat sciatic nerves[J].Journal of Southern Medical University,2004,(07):85.
[4]郭倩倩,刘志燕,姜丽丽,等.营养缺乏对人肺癌细胞自噬的诱导作用[J].南方医科大学学报,2014,(05):627.
[5]王玲,余乐,古春萍,等.稳定表达Ras的人成纤维细胞改变自噬活性的细胞效应[J].南方医科大学学报,2014,(11):1578.
[6]李良庆,谢文焌,潘敦.巴弗洛霉素A1抑制胃癌MGC-803细胞增殖及增强奥沙利铂的敏感性[J].南方医科大学学报,2015,(10):1400.
[7]叶红伟,方婷婷,谷小雨,等.Rho激酶和自噬在法舒地尔抗心肌缺血/再灌注损伤中的作用[J].南方医科大学学报,2016,(12):1706.
[8]吕梦,陆航.Beclin1基因在SW620细胞自噬和凋亡中作用[J].南方医科大学学报,2017,(03):373.
[9]郭伟洪,陈昭宇,陈豪,等.丝胶蛋白通过自噬通路调控人胃癌MKN45细胞的增殖[J].南方医科大学学报,2018,(02):148.
[10]赖华毅,陈强,李红,等.p38MAPK信号通路在鼠伤寒沙门菌spvB基因影响Henle-407细胞自噬中的作用[J].南方医科大学学报,2018,(03):268.

备注/Memo

备注/Memo:
收稿日期:2004-6-20。
基金项目:国家自然科学基金(30300191;39870382);广东省自然科学基金(032898)
作者简介:朴英杰(1931- ),男,现为第一军医大学专家组成员,教授,博士生导师.主要研究领域:组织胚胎学和电子显微镜学,尤其是溶酶体消化和自噬方面.学术兼职有:国际组织细胞化学联合会中方秘书、《Acta Histochemistry and Cytocheistry》编委,曾任中国电子显微镜学会理事、广东省电子显微镜学会理事长,电话:020-61648174
更新日期/Last Update: 1900-01-01